非定向激活沉默基因簇挖掘链霉菌活性次级代谢产物研究进展  

Advances in non-directed activation of silent gene clusters for mining active secondary metabolites of Streptomyces

作　　者：宋国栋 廖宏娟 张志斌[1] 朱笃[1,2] SONG Guo-dong;LIAO Hong-juan;ZHANG Zhi-bin;ZHU Du(Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province,Jiangxi Normal University,Nanchang 330022,China;Key Lab of Bioprocess Engineering of Jiangxi Province,College of life sciences,Jiangxi Science and Technology Normal University,Nanchang 330013,China)

机构地区：[1]江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,南昌330022 [2]江西科技师范大学生命科学学院,江西省生物加工过程重点实验室,南昌330013

出　　处：《天然产物研究与开发》2023年第8期1442-1456,共15页Natural Product Research and Development

基　　金：国家自然科学基金(82160671)。

摘　　要：链霉菌(Streptomyces spp.)是活性次级代谢产物的主要生产菌,在发现结构新颖化合物上具有重大潜力。随着对链霉菌基因组数据的深入分析,发现大量产次级代谢产物合成基因簇处于沉默状态,因此利用非定向和定向策略激活链霉菌中沉默基因簇、挖掘结构新颖化合物成为当前的主要手段。本文主要综述了培养基组成改变或培养条件优化、共培养、添加化学激发子及全局性调控等非定向策略在挖掘链霉菌次级代谢产物中的应用以及取得的进展,以期为链霉菌次级代谢产物高效开发提供参考。Streptomyces are major producers of active secondary metabolites and show great potential for discovering structurally novel compounds.In-depth analysis of Streptomyces genomic data revealed that a large number of gene clusters producing secondary metabolites were silenced.Therefore,the use of undirected and directed strategies to activate silenced gene clusters in Streptomyces has become a major means of mining structurally novel compounds.In this review,we reviewed the application and progress of non-directed strategies in secondary metabolite mining of Streptomyces,such as changing medium composition or optimizing culture conditions,co-culture,adding chemical elicitors and global regulation,in order to provide reference for the efficient development of secondary metabolites in Streptomyces.

关 键 词：链霉菌 次级代谢产物 沉默基因簇 共培养 激发子 

分 类 号：Q936[生物学—微生物学]