黄精的炮制工艺优化及抗衰老作用研究  

Study on the optimal processing-technology and anti-aging effects of Polygonatum sibiricum

作　　者：沈灵 曾琳琳 骆巧媚 范依霖 周霞[1] 万军[1] SHEN Ling;ZENG Linlin;LUO Qiaomei;FAN Yilin;ZHOU Xia;WAN Jun(College of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan,610031 P.R.China)

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《华西药学杂志》2023年第4期394-398,共5页West China Journal of Pharmaceutical Sciences

摘　　要：目的优选黄精酒蒸工艺及其抗衰老作用的研究。方法以黄精多糖、5-羟甲基糠醛、醇浸出物为评价指标,润制、蒸制、焖制的时间为影响因素,采用单因素实验正交试验优选酒蒸工艺;建立小鼠衰老模型,测定小鼠的体重、肝脾指数、血清及脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的活性水平,评价炮制后黄精体内抗氧化能力的变化。结果优选的黄精炮制工艺为:取生黄精100 g,加黄酒20 g,润制1 h,蒸制7 h,焖制3 h,于60~80℃干燥;酒黄精在升高脾脏指数、SOD和GSH-Px的活性,降低肝脏指数和MDA的活性,改善D-半乳糖诱导衰老小鼠的体重减轻和生理状态等方面优于生黄精。结论优选的黄精酒蒸工艺稳定、可行,酒黄精较生黄精在抗氧化、抗衰老方面作用更佳。OBJECTIVE To optimize the wine-steaming process and explore the anti-aging effects of Polygonatum sibiricum(PS).METHODS The process for wine-steaming was optimized by the content of polysaccharides,5-hydroxymethyl furfural and alcohol extracts as indicators.By setting the soaking time,steaming time and stewing time as levels,orthogonal test was used to optimize wine-steaming process of PS on the basis of single factor test.The aging model of mice was established,and the changes of body weight,liver and spleen indexes,superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)activities in serum and brain tissues were measured to evaluate the changes of antioxidant capacity of PS after processing.RESULTS The optimal process was as follows taking 100 g of raw PS,adding 20 g of wine,soaking 1 h,steaming 7 h,stewing 3 h,and drying at 60-80℃.Wine-steaming PS(WPS)outperformed PS in improving body weight and mental state by increasing the spleen index,SOD and GSH-Px activities,decreasing the liver index and MDA activities in D-galactose-induced aging mice.CONCLUSION The optimal process of WPS is stable and feasible.WPS outperforms RPS in antioxidant and anti-aging effects.

关 键 词：黄精 炮制 酒蒸 工艺优选 抗氧化酶 肝脏指数 脾脏指数 抗衰老 

分 类 号：R917[医药卫生—药物分析学] R96[医药卫生—药学]