GDF11通过减轻炎症反应缓解主动脉瘤形成  

GDF11 alleviates the formation of aortic aneurysm by reducing inflammatory responses

作　　者：薛超 李步潆 江丽青 王伟光 金振晓 段维勋 任恺 XUE Chao;LI Buying;JIANG Liqing;WANG Weiguang;JIN Zhenxiao;DUAN Weixun;REN Kai(Department of Cardiovascular Surgery,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]空军军医大学西京医院心血管外科,陕西西安710032

出　　处：《空军军医大学学报》2023年第8期712-716,共5页Journal of Air Force Medical University

基　　金：国家自然科学基金(82070503,82270420,82241204);陕西省重点产业创新链(群)-社会发展领域项目(2022ZDLSF02-01);陕西省创新能力支撑计划项目(2021PT-019);陕西省自然科学基础研究计划青年项目(2021JQ-347)。

摘　　要：目的探讨生长分化因子(GDF11)缓解主动脉瘤形成的保护作用及其对炎症反应的影响。方法雄性3周龄C57BL/6小鼠60只,体质量10~13 g,随机分成3组:正常对照组20只、动脉瘤组20只、动脉瘤与GDF11共处理组20只。利用β氨基丙腈(BAPN)饮水法构建小鼠动脉瘤模型,剂量为1 g·kg^(-1)·d^(-1),4周后检测主动脉血管直径,GDF11、MMP9蛋白表达和炎症因子表达水平。结果给予BAPN喂养后,小鼠主动脉血管直径扩大,GDF11蛋白表达下降,而MMP9蛋白表达和炎症因子表达水平升高。给予GDF11蛋白可显著缓解主动脉血管直径扩大,下调MMP9蛋白表达和炎症因子表达水平(P<0.01)。结论GDF11共处理可显著缓解主动脉血管扩张,减轻主动脉血管炎症反应。Objective To investigate the protective effect of GDF11 on the formation of aortic aneurysm and its effect on inflammatory responses.Methods Sixty 3-week-old male C57BL/6 mice(1013 g)were randomly divided into three groups:normal control group(n=20),aneurysm group(n=20)and aneurysm and GDF11 co-treatment group(n=20).A mouse aneurysm model was constructed withβ-aminopropionitrile(BAPN)drinking water,at a dose of 1 g·kg^(-1)·d^(-1).The diameter of aortic vessels,GDF11 and MMP9 protein expression and experession levels of inflammatory factors were detected after 4 weeks.Results After feeding with BAPN,the diameter of aortic vessels enlarged,GDF11 protein expression decreased,while MMP9 protein expression and expression levels of inflammatory factors increased.GDF11 could significantly alleviate the enlargement of aortic diameter,and down-regulate MMP9 protein expression and expression levels of inflammatory factors(P<0.01).Conclusion GDF11 co-treatment can significantly alleviate aortic vasodilation and reduce aortic vascular inflammation.

关 键 词：主动脉瘤 GDF11 β氨基丙腈 炎症反应 

分 类 号：R543.16[医药卫生—心血管疾病]