维生素D对辛酸高脂饮食引起的前列腺癌小鼠骨转移的影响  

Effect of vitamin D on bone metastasis of prostate cancer mice induced by octanoic acid and high-fat diet

作　　者：赵海军[1] 李伟[1] 魏瑜[1] 张丽翠[1] 张玉[1] 王新敏[1] 王翠喆 ZHAO Haijun;LI Wei;WEI Yu;ZHANG Licui;ZHANG Yu;WANG Xinmin;WANG Cuizhe(The First Affiliated Hospital of Shihezi University,Shihezi 832000,China;不详)

机构地区：[1]石河子大学第一附属医院,新疆石河子832000 [2]石河子大学医学院,新疆石河子832000

出　　处：《实用医学杂志》2023年第14期1756-1761,共6页The Journal of Practical Medicine

基　　金：兵团指导性科技计划(编号:2022ZD083);石河子大学科研项目(编号:ZZZC201817A)。

摘　　要：目的研究维生素D治疗对辛酸高脂饮食引起的前列腺癌小鼠骨转移的影响。方法建立前列腺癌小鼠骨转移模型,将其分为正常喂养的Control组、高脂饲料喂养的High fat组、添加辛酸的高脂饲料喂养的Bitter组以及维生素D治疗的辛酸高脂饲料喂养的Vitamin D组。研究结束后,取3组小鼠外周血检测总胆固醇、甘油三酯、低密度脂蛋白、钙和25羟维生素D(25-OH-D)含量,检测各组小鼠骨转移程度及肿瘤组织E-cadherin和p38蛋白表达情况,体外使用Transwell小鼠研究维生素D对辛酸影响的RM-1细胞侵袭迁移能力的影响。结果与Control组相比,High fat组和Bitter组小鼠血液中总胆固醇、甘油三酯、低密度脂蛋白及钙、^(18)F-NaF PET-CT检测结果中SUV_(min)、SUV_(max)和SUV_(mean)均显著升高(P<0.05);并且Bitter组显著高于High fat组(P<0.05),而Vitamin D组显著低于Bitter组(P<0.05)。在体外,辛酸处理显著提高RM-1细胞的侵袭和迁移能力,但维生素D可抑制辛酸促进的RM-1细胞的侵袭和迁移能力。在体内,辛酸可显著提高高脂饮食前列腺癌骨转移小鼠肿瘤组织中E-cadherin和p-p38/p38蛋白表达,但维生素D可显著降低辛酸增加的E-cadherin和p-p38/p38蛋白表达水平。结论维生素D治疗抑制辛酸高脂饮食促进的前列腺癌小鼠骨转移,其机制可能与增加血清25-OH-D含量和抑制癌细胞中E-cadherin、p-p38/p38蛋白表达有关。Objective To study the effect of vitamin D treatment on bone metastasis of prostate cancer mice induced by octanoic acid and high-fat diet.Methods The bone metastasis model of prostate cancer mice was established,and the mice were divided into normal feeding Control group,High fat group fed with high fat diet,Bitter group fed with caprylic acid and Vitamin D group fed with caprylic acid and high fat diet.After the study,the contents of total cholesterol,triglyceride,low-density lipoprotein,calcium and 25-hydroxyvitamin D(25-OH-D)in peripheral blood of mice in the three groups were detected,and the degree of bone metastasis and the expression of E-cadherin and p38 protein in tumor tissues of mice in each group were detected.Transwell mice were used in vitro to study the effect of vitamin D on the invasion and migration ability of RM-1 cells affected by caprylic acid.Results Compared with the control group,the contents of total cholesterol,triglyceride,low density lipoprotein and calcium in the blood of mice in the High fat group and Bitter group,as well as the contents of SUV_(min),SUV_(max) and SUV_(mean) in the ^(18)F-NaF PET-CT test results were significantly increased(P<0.05);And Bitter group was significantly higher than High fat group(P<0.05),while Vitamin D group was significantly lower than Bitter group(P<0.05).In vitro,octanoic acid treatment significantly increased the invasion and migration ability of RM-1 cells,but vitamin D could inhibit the invasion and migration ability of RM-1 cells promoted by octanoic acid.In vivo,caprylic acid can significantly increase the expression of E-cadherin and p-p38/p38 protein in tumor tissue of mice with bone metastasis of prostate cancer from high-fat diet,but vitamin D can significantly reduce the increased E-cadherin and p-p38/p38 protein table of caprylic acid.Conclusion Vitamin D treatment inhibited bone metastasis of prostate cancer mice promoted by octanoic acid and high-fat diet,and its mechanism may be related to the increase of serum 25-OH-D content and the

关 键 词：维生素D 辛酸 高脂肪 前列腺癌 骨转移 小鼠 

分 类 号：R339.21[医药卫生—人体生理学]