花溪盾孢囊霉:一个发现于中国贵阳的丛枝菌根真菌新种  

Cetraspora huaxica,a new species of arbuscular mycorrhizal fungi(Glomeromycotina)from Guiyang,China

作　　者：陈德瑶 龙春丽 何荣健 董芮豪 江龙 CHEN Deyao;LONG Chunli;HE Rongjian;DONG Ruihao;JIANG Long(Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,Guizhou,China;Tongren Polytechnic College,Tongren 554300,Guizhou,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院、山地植物资源保护与保护种质创新教育部重点实验室,贵州贵阳550025 [2]铜仁职业技术学院,贵州铜仁554300

出　　处：《菌物学报》2023年第8期1661-1672,共12页Mycosystema

基　　金：国家科技支撑项目(2015BAD04B0204);贵州梵净山国家级自然保护区管理局项目(梵管局[科]2019-GD-AM)。

摘　　要：本研究从贵阳市花溪区的金佛山方竹根际土壤中分离得到盾孢囊霉属的一个新物种:花溪盾孢囊霉。孢子透明至近透明,大小为187–361×210–378μm,产孢细胞为浅黄色至淡黄棕色;发芽盾室透明至淡黄棕色,具有4–8个裂片;孢子壁有3层:3层外壁层(OWL1-3),2层中壁层(MWL1-2)和3层内壁层(IWL1-3),OWL2与IWL3在Melzer’s试剂中呈深粉色到亮红棕色。基于核rDNA序列[覆盖部分SSU(small subunit)、整个ITS(internal transcribed spacer)和部分LSU(large subunit)区段;SSU-ITS-LSU]的系统发育分析表明,该种位于盾孢囊霉属,且形成独立分支。本研究对该种进行了详细的形态描述和特征图示,并讨论了与其近缘种的鉴别特征。Cetraspora huaxica,a new species of Cetraspora(Diversisporales,Glomeromycotina),was isolated from rhizosphere soil of Chimonobambusa utilis in Huaxi District of Guiyang City,China.It forms hyaline spores 187–361×210–378μm on pale yellow to pale yellow brown sporogenous cells.The germination shields are hyaline to pale yellow brown with multiple(4–8)lobes;the spores have three walls:a triple-layered outer wall(OWL1-3),a bi-layered middle wall(MWL1-2)and a triple-layered inner wall(IWL1-3),of which the OWL2 and IWL3 stain deep pink to bright red-brown when exposed in Melzer’s reagent.Phylogenetic analyses based on the sequences of nuclear rDNA(spanning the partial small subunit,whole internal transcribed spacer,and partial large subunit segment;SSU-ITS-LSU)indicate that this species belongs to the genus Cetraspora and forms an independent clade.Detailed descriptions of the new taxon and a comparison with its phylogenetically related taxa are provided.

关 键 词：丛枝菌根真菌 形态学 SSU-ITS-LSU 分类 

分 类 号：Q949.32[生物学—植物学]