基于Py-GC/MS分析黑木耳新品种‘农黑2号’木耳黑色素的热裂解产物  

Pyrolysis products of melanin from Auricularia heimuer new cultivar‘Nonghei No.2’analyzed by Py-GC/MS

作　　者：李晓敏 谢颂钰 陶永新[2,3] 吴小平[1,2] 傅俊生 LI Xiaomin;XIE Songyu;TAO Yongxin;WU Xiaoping;FU Junsheng(College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学菌物研究中心,福建福州350002 [3]福建农林大学园艺学院,福建福州350002

出　　处：《菌物学报》2023年第8期1766-1774,共9页Mycosystema

基　　金：福建省种业创新与产业化工程项目(fjzycxny2017010);食用菌资源开发和高效加工关键技术研究(2018YFD0400200)。

摘　　要：利用热裂解-气相色谱质谱联用(pyrolysis gas chromatography mass spectrometry,Py-GC/MS)分析技术测定黑木耳新品种‘农黑2号’黑色素裂解产物组分的种类及含量,为解析黑色素的结构奠定理论基础。本研究通过碱溶酸沉法提取黑色素,并用紫外可见吸收光谱、傅立叶红外光谱、有机元素分析鉴定黑色素,采用Py-GC/MS技术分析其热裂解产物。结果表明,‘农黑2号’黑色素的最大吸收峰值在波长220 nm处,傅立叶红外光谱符合典型的黑色素特征;经有机元素分析,C、H、N、O元素的含量分别为43.47%、5.24%、11.71%和39.58%,不含硫元素,为真黑色素;检出的热裂解产物主要有苯类、苯酚类、吲哚类、苯基腈类、烃类、酸类等物质,其中苯、苯酚、吲哚等化合物为真黑色素的裂解产物。综上所述,‘农黑2号’黑色素为真黑色素,其基本骨架为苯环和吲哚,本研究为精准解析黑木耳黑色素结构提供科学依据。The types and content of melanin cleavage of the new cultivar of Auricularia heimuer‘Nonghei No.2’were determined by pyrolysis gas chromatography mass spectrometry(Py-GC/MS)analysis technology for analysing the structure of melanin.Melanin was extracted by alkaline acid precipitation method,and identified by using ultraviolet-visible absorption spectroscopy,Fourier infrared spectroscopy and organic element analysis.The thermal cracking products were analyzed by Py-GC/MS technology.The results show that the maximum absorption peak of melanin of‘Nonghei No.2’is at a wavelength of 220 nm,and the Fourier infrared spectrum conforms with the typical melanin characteristics.Organic element analysis indicates that the contents of C,H,N and O elements are 43.47%,5.24%,11.71%and 39.58%,respectively.The melanin does not contain sulfur element,which is eumelanin.The detected thermal cracking products are mainly benzene,phenol,indole,phenylnitrile,hydrocarbons,acids and other substances,among which benzene,phenol,indole and other compounds are the cleavage products of eumelanin.In summary,‘Nonghei No.2’melanin is eumelanin,and its basic skeleton is benzene ring and indole.

关 键 词：黑木耳新品种‘农黑2号’ 黑色素 真黑色素 热裂解气相色谱-质谱法(Py-GC/MS) 

分 类 号：S646.6[农业科学—蔬菜学]