脊髓损伤中铁死亡相关基因的生物信息学分析  

Bioinformatics analysis of iron death related genes in spinal cord injury

作　　者：寇红伟[1] 闫立言 韩萧男 张明康 尚国伟[1] 尚春风 冷子宽 姬彦辉 程田[1] 刘宏建[1] Kou Hongwei;Yan Liyan;Han Xiaonan;Zhang Mingkang;Shang Guowei;Shang Chunfeng;LengZikuan;Ji Yanhui;Cheng Tian;Liu Hongjian(Department of Orthopaedics,the First Affiliated Hospital of Zhengzhou University,Zhengzhou450052,China)

机构地区：[1]郑州大学第一附属医院骨科,郑州450052

出　　处：《中华实验外科杂志》2023年第7期1269-1271,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨脊髓损伤(SCI)中铁死亡的相关分子机制并分析其生物学功能。方法从基因表达(GEO)数据库搜索并下载SCI相关数据组表达基因芯片数据GSE183591, 从FerrDb数据库下载铁死亡相关基因, 使用Network Analysis分析GSE183591获得差异表达基因(DEGs), 对数据取交集获得铁死亡相关差异表达基因(FRDEGs), 使用David工具进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)分析, 使用STRING网站构建蛋白与蛋白相互作用网络并通过cytoHubba确定关键基因, 使用CIBERSORT包进行22种免疫细胞浸润分析, 使用Timer 2.0工具进行9种免疫浸润细胞分析。结果共识别42个在1、2、8周共同表达的FRDEGs。GO和KEGG分析结果发现FRDEGs主要在免疫和炎性反应方面富集。STRING分析结果显示, 共有42个节点, 71条边, cytoHubba分析确认了5个中枢调控基因, Toll样受体4(Tlr4)、白细胞介素-1β(IL1b)、转化生长因子1(Tgfb1)、血红素加氧酶1(Hmox1)、Cd44。CIBERSORT和Timer 2.0发现在不同的时间点, 巨噬细胞和树突状细胞的表达差异均有统计学意义。结论 Tlr4、IL1b、Tgfb1、Hmox1、Cd44是SCI后铁死亡的主要调控基因, 可能通过免疫反应和炎性反应影响SCI的发生发展。Objective To explore the molecular mechanism of iron death in spinal cord injury(SCI)and analyze its biological function.Methods SCI related data set expression gene chip data GSE183591 were searched and downloaded from gene expression omnibus(GEO)database,and iron death related genes were downloaded from FerrDb database.Network Analysis was used to analyze GSE183591 to obtain differentially expressed genes(DEGs).Data overlap was taken to obtain iron death related differenti-ally expressed genes(FRDEGs).David website was used for gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)analysis.The protein-protein interaction network was constructed by STRING website and the key genes were identified by cytoHubba.A total of 22 kinds of immune cell infil-tration were analyzed by CIBERSORT package and 9 kinds of immune infiltrating cells were analyzed by Timer 2.0 tool.Results A total of 42 FRDEGs co-expressed at 1 week,2 weeks and 8 weeks were identi-fied.GO and KECG analysis showed that FRDEGs were mainly enriched in immune and inflammatory reactions.STRING analysis showed that there were 42 nodes and 71 edges.CytoHubba analysis confirmed 5 central regulatory genes[toll like receptor 4(Tlr4),interleukin 1 beta(ILib),transforming growth fac-tor beta 1(Tgfb1),heme oxygenase 1(Hmoxl),and Cd44].CIBERSORT and Timer 2.0 found that there were significant differences in the expression of macrophages and dendritic cells at different time points.Conclusion Tlr4,ILIb,Tgfbl,Hmoxl and Cd44 are the main regulatory genes of iron death after SCI,which may affect the occurrence and development of SCI through immune response and inflammatory response.

关 键 词：铁死亡 脊髓损伤 免疫细胞 

分 类 号：R651.2[医药卫生—外科学] Q811.4[医药卫生—临床医学]