杂交兰‘红美人’×寒兰‘杨贵妃’杂交后代增殖与分化研究  

Study on the Proliferation and Differentiation of Rhizomes in the Hybrid Progeny of Hybrid Orchid‘Hongmeiren’X Cymbidium kanran‘Yangguifei'

作　　者：杨松敏 唐若楠 程梦雅 牛沐奇 陈和明 彭东辉[1,2,3] YANG Songmin;TANG Ruonan;CHENG Mengya;NIU Muqi;CHEN Heming;PENG Donghui(College of Landscape Architecture and Arts,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;Fujian Engineering Research Center of Ornamental Plant Germplasm Resources Innovation and Application,Fuzhou,Fujian 350002;Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;Environmental Horticulture Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization,Guangzhou,Guangdong 510640)

机构地区：[1]福建农林大学风景园林与艺术学院,福建福州350002 [2]福建省观赏植物种质资源创新与应用工程技术研究中心,福建福州350002 [3]福建农林大学兰科植物保护与利用国家林草局重点实验室,福建福州350002 [4]广东省农业科学院环境园艺研究所,广东省园林花卉种质创新综合利用重点实验室,广东广州510640

出　　处：《北方园艺》2023年第14期63-70,共8页Northern Horticulture

基　　金：国家自然科学基金资助项目(2019YFD1001000)。

摘　　要：以杂交兰‘红美人’×寒兰‘杨贵妃’杂交后代的根状茎为试材,采用L9(34)正交实验方法,研究了6-BA、NAA、TDZ、不同光周期及不同基本培养基对根状茎增殖、叶芽分化以及花芽分化的影响,以期探究杂交兰根状茎增殖与分化适合的条件。结果表明:在1.0 g·L^(-1)花宝1号+1.0 g·L^(-1)花宝2号+1.0 mg·L^(-1)TDZ+1.0 mg·L^(-1)NAA+0.1 g·L^(-1)活性炭+40.0 g·L^(-1)蔗糖+7.0 g·L^(-1)琼脂+6.0 g·L^(-1)蛋白胨+40.0 g·L^(-1)香蕉+40.0 g·L^(-1)马铃薯处理中,根状茎的增殖效果最好;光周期12 h/12 h(光照/黑暗)的增殖系数最高,10 h/14 h(光照/黑暗)的生长速度最快;在2.0 g·L^(-1)花宝1号+1.0 g·L^(-1)花宝2号+5.0 mg·L^(-1)6-BA+1.0 mg·L^(-1)NAA+0.1 g·L^(-1)活性炭+40.0 g·L^(-1)蔗糖+7.0 g·L^(-1)琼脂+6.0 g·L^(-1)蛋白胨+50.0 mg·L^(-1)椰汁的处理中,叶芽分化率最优;在基本培养基MS+5.0 mg·L^(-1)6-BA+1.0 mg·L^(-1)NAA+0.1 g·L^(-1)活性炭+40.0 g·L^(-1)蔗糖+7.0 g·L^(-1)琼脂+6.0 g·L^(-1)蛋白胨+40.0 g·L^(-1)香蕉+40.0 g·L^(-1)马铃薯的处理中,根状茎产生了花芽分化。Taking hybrid orchid‘Hongmeiren'X Cymbidium kanran‘Yangguifei'of rhizomes as experimental material,using Lg(3*)orthogonal experiment method,the effects of 6-BA,NAA,TDZ on different photoperiod and basic culture medium of rhizomes proliferation,leaf bud differentiation and flower bud differentiation were studied,in order to explore the hybrid laingen stems proliferation and differentiation of suitable conditions.The results showed that the best formula of rhizome proliferation,2.0 g·L^(-1)Huabao 1+1.0 g·L^(-1)Huabao 2+1.0 mg·L^(-1)TDZ+1.0 mg·L^(-1)NAA+0.1 g·L^(-1)activated carbon+40.0 g·L^(-1)sucrose+7.0 g·L^(-1)agar+6.0 g·L^(-1)proteinpeptone+40.0 g·L^(-1)banana+40.0 g·L-'potato;the proliferation coefficient was highest at 12 hours/12 hours(light/dark)and the fastest growth rate at 10 hours/14 hours(light/dark).The best formula of leaf bud differentiation,2.0 g·L^(-1)Huabao 1+1.0 g·L^(-1)Huabao 2+5.0 mg·L^(-1)6-BA+1.0 mg·L^(-1)NAA+0.1 g·L^(-1)activated carbon+40.0 g·L^(-1)sucrose+7.0 g·L^(-1)agar+6.0 g·L-'peptone+50.0 mg·L^(-1)coconut milk.In MS+5.0 mg·L^(-1)6-BA+1.0 mg·L^(-1)NAA+0.1 g·L^(-1)activated carbon+40.0 g·L^(-1)sucrose+7.0 g·L^(-1)agar+6.0 g·L-I proteinpeptone+40.0 g·L^(-1)banana+40.0 g·L^(-1)potato,the rhizome produced flower buds.

关 键 词：杂交兰 组织培养 根状茎 增殖 分化 

分 类 号：S682.3[农业科学—观赏园艺]