基于ISSR分子标记的贵州冬荪遗传多样性分析  

Genetic Diversity Analysis of Phallus impudicus Strains Based on ISSR Molecular Markers

作　　者：卢颖颖 刘宏宇 黄万兵 桂阳[1,2,3,4] 朱国胜 LU Yingying;LIU Hongyu;HUANG Wanbing;GUI Yang;ZHU Guosheng(Institute of Crop Germplasm Resources,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006;Guizhou Key Laboratory of Edible Fungi Breeding,Guiyang,Guizhou 550006;Resource Breeding Function Laboratory of Guizhou Modern Agricultural Industry Technology System of Edible Fungi,Guiyang,Guizhou 550006;Resource Breeding Laboratory of Guizhou Engineering Research Center of Edible Fungi,Guiyang,Guizhou 550006)

机构地区：[1]贵州省农业科学院贵州省农作物品种资源研究所,贵州贵阳550006 [2]贵州省食用菌育种重点实验室,贵州贵阳550006 [3]贵州省食用菌现代农业产业技术体系资源育种功能实验室,贵州贵阳550006 [4]贵州省食用菌工程技术研究中心资源育种实验室,贵州贵阳550006

出　　处：《北方园艺》2023年第14期133-139,共7页Northern Horticulture

基　　金：贵州省科技计划资助项目(黔科合重大专项字[2019]3008,黔科合平台人才[2019]5105号,黔科合支撑[2019]2451-6,黔科合成果[2023]一般065,黔科合基础-ZK[2023一般172]);贵州省科技支撑计划资助项目(黔科合支撑[2022]重点025号);贵州省食用菌现代农业产业技术体系资助项目(GZCYTX2021-GZCYTX2025)。

摘　　要：以不同地区的20份冬荪种质资源为试材,利用ISSR(inter-simple sequence repeat简单重复序列间扩增)分子标记技术,从78条引物中筛选出条带清晰、重复性高和特异性强的引物,在供试冬荪菌株中进行扩增。利用NTSYS 2.10e聚类分析软件UPGMA法进行聚类分析,同时利用PopGen 32软件对20份冬荪种质ISSR统计结果进行遗传多样性分析,以期通过分子标记技术评价冬荪种质资源遗传多样性,为冬荪遗传育种提供优质材料。结果表明:筛选出具备多态性ISSR引物24条,对供试冬荪菌株进行PCR扩增共获得谱带有788条,其中多态性谱带768条,多态位点比例达到97.5%;遗传多样性分析显示平均等位基因(Na)为1.9913,平均有效等位基因数(Ne)为1.5856,平均Nei′s基因多样性指数(He)为0.3438,平均Shannon信息指数(I)为0.5161;在遗传相似系数为0.67时,20份冬荪菌株可分为三大类群。综上,20株冬荪种质资源间具有丰富的遗传多样性,亲缘关系较复杂;不同来源的种质由于菌种流通频繁而导致亲缘关系复杂,育种亲本的选择纯化周期较长。The genetic diversity of 20 Phallus impudicus species from different regions was analyzed using Inter-simple sequence repeat(ISSR)molecular markers.From 78 primers,the primers with clear bands,high repeatability and high specificity were screened and amplified in the tested strains of Phallus impudicus.The UPGMA method of NTSYS 2.10e cluster analysis software was used for cluster analysis,and the ISSR statistics of 20 Phallus impudicus samples were analyzed by PopGen32,in order to evaluate the genetic diversity of Phallus impudicus germplasm resources by molecular markers and provide high-quality materials for genetic breeding.The results showed that a total of 24 ISSR primers with polymorphism were screened out,and 788 ISSR bands were amplified by PCR,included 768 polymorphism bands,with the proportion of polymorphic loci reaching 97.5%;genetic diversity analysis showed that the average allele(Na)was 1.9913,the average effective allele number(Ne)was 1.5856,the average Nei's gene diversity index(He)was 0.3438,and the average Shannon information index(1)was 0.5161.When the genetic similarity coefficient was 0.67,the 20 strains could be divided into three groups.In conclusion,there were abundant genetic diversity and complex genetic relationship among the 20 species.Due to the frequent circulation of germplasm from different sources,the genetic relationship was complicated,and the selection and purification cycle of breeding parents was long.

关 键 词：冬荪 ISSR 遗传多样性 

分 类 号：S646[农业科学—蔬菜学]