接绪资冲颗粒对实验性自身免疫性甲状腺炎致卵巢储备功能下降小鼠肠道菌群的影响  

Effects of Jiexu Zichong Granules on intestinal fora in mice with decreased ovarian reserve caused by experimental autoimmune thyroiditis

作　　者：李方远[1] 陆华[1,2] 王通 苏先芝 张琦[1,2] 黎欣韵 刘芊辰 LI Fangyuan;LU Hua;WANG Tong;SU Xianzhi;ZHANG Qi;LI Xinyun;LIU Qianchen(Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Hospital of Chengdu University of TCM,Chengdu 610075,China;Zhumadian Hospital of Traditional Chinese Medicine,Zhumadian 463000,China)

机构地区：[1]成都中医药大学,成都610075 [2]成都中医药大学附属医院,成都610075 [3]驻马店市中医院,驻马店463000

出　　处：《中华中医药杂志》2023年第8期3799-3804,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点研发计划中医药现代化研究(No.2018YFC1704305);国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者(No.国中医药人教函[2018]284号);四川省中医药院士后备人才培养项目(No.川中医药函[2020]73号)。

摘　　要：目的:探讨接绪资冲颗粒对实验性自身免疫性甲状腺炎(EAT)导致的卵巢储备功能下降小鼠肠道菌群的影响。方法:30只性成熟昆明雌鼠随机分为对照组、模型组和接绪资冲颗粒组(ZCWS组),各10只。抗原免疫诱导配合高碘水喂养13周建立EAT导致的卵巢储备功能下降小鼠模型,对照组采用PBS皮下多点注射。于造模的7周开始,对照组与模型组予以0.9%氯化钠溶液灌胃(0.3 mL/d),ZCWS组采用0.64 g/mL的中药混悬液灌胃(0.3 mL/d),连续7周。采用HE染色进行甲状腺病理评分和各级卵泡计数,ELISA检测甲状腺球蛋白抗体(TGAb)、甲状腺过氧化物酶抗体(TPOAb),16S rDNA测序技术分析3组肠道菌群变化。结果:接绪资冲颗粒可改善甲状腺病理评分和卵巢储备(P<0.01),调节模型小鼠肠道菌群相对丰度:显著升高杆菌纲、乳杆菌(科、目、属)、Christensenellaceae_R-7_group、uncultured_bacterium_g_Christensenellaceae_R-7_group菌种相对丰度(P<0.05,P<0.01);显著降低厚壁菌纲、γ-变形菌纲、肠杆菌科、肠杆菌目、埃希氏杆菌属、escherichia_coli菌种、芽胞杆菌目、Prevotellaceae_NK3B31_group、Prevotellaceae UCG-001、bacteroides xylanisolvens菌种、Veillonella_parvula菌种相对丰度(P<0.05,P<0.01)。结论:接绪资冲颗粒可能通过调节肠道菌群改善EAT小鼠的卵巢储备功能。Objective:To observe the effect of Jiexu Zichong Granules on the intestinal flora of mice with decreased ovarian reserve(DOR)caused by experimental autoimmune thyroiditis(EAT),and to explore its mechanism.Methods:Thirty female sexually mature Kunming mice were divided into control group,model group and Jiexu Zichong Granules(ZCwS)group,10 mice in each group.The DOR mouse model induced by EAT was established by antigen immunization induction combined with high iodine water feeding for 13 weeks,and the control group was subcutaneously injected with PBS at multiple points.Beginning from the 7th week of modeling,the control group and model group were gavaged with normal saline(0.3 mL/d),and the ZCWS group was gavaged with 0.64 g/mL Chinese medicine suspension(0.3 mL/d)for 7 consecutive weeks.HE staining was used to score thyroid pathology and count follicles at all levels,ELISA was used to detect TPOAb and TGAb,and 16S rDNA sequencing technology was used to analyze the changes of intestinal flora in the three groups.Results:Jiexu Zichong Granules can improve thyroid pathological score and ovarian reserve(P<0.01).Jiexu Zichong Granules could regulate the relative abundance of intestinal flora in model mice:the relative abundances of Bacillus,Lactobacillus(family,genus,species),Christensenellaceae_R-7_group,uncultured_bacterium_g_Christensenellaceae_R-7_group were significantly increased(P<0.05,P<0.01);the relative abundances of Firmicutes,-Proteobacteria,Enterobacteriaceae,Enterobacteriales,Escherichia,escherichia-coli,Bacillus,Prevotellaceae_NK3B31_group,Prevotellaceae UCG-001,bacteroidesxylanisolvens,Veillonella_parvula were significantly reduced(P<0.05,P<0.01).Conclusion:Jiexu Zichong Granules may improve ovarian reserve in EAT micebyregulating intestinal flora.

关 键 词：实验性自身免疫性甲状腺炎 肠道菌群 卵巢储备 接绪资冲颗粒 

分 类 号：R285.5[医药卫生—中药学]