基于血清蛋白质组学分析桥本甲状腺炎合并肥胖患者血清蛋白质差异表达  

Analysis of differentially expressed proteins in Hashimoto thyroiditis patients with obesity by serum-based proteomics

作　　者：马国英 加孜热亚·再依拿提 李素丽 郭艳英[1,2,3] MA Guoying;Jiazireya·ZAIYINATI;LI Suli;GUO Yanying(Xinjiang Clinical Research Center for Diabetes,Urumqi 830001;Xinjiang Endocrinology Diabetes Institute,Urumqi 830001;Department of Endocrinology,People′s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China)

机构地区：[1]新疆糖尿病临床医学研究中心,新疆乌鲁木齐830001 [2]新疆内分泌糖尿病研究所,新疆乌鲁木齐830001 [3]新疆维吾尔自治区人民医院内分泌科,新疆乌鲁木齐830001

出　　处：《基础医学与临床》2023年第9期1375-1382,共8页Basic and Clinical Medicine

基　　金：新疆维吾尔自治区自然科学基金(2020D01C130)。

摘　　要：目的探讨体质量对桥本甲状腺炎(HT)患者中肥胖与正常体质量对血清中蛋白质表达差异的影响,探寻存在的差异蛋白质与相关通路。方法收集桥本甲状腺炎患者中肥胖与正常体质量患者血清各10例,健康对照组血清10人,提取血清中蛋白质,使用胰蛋白酶降解,进行液相色谱-质谱联用分析,与对照组对比分析,鉴定两组差异蛋白质的表达变化,并进行生物信息学分析。结果检测到正常体质量的桥本甲状腺炎组与对照组的血清差异蛋白质共有180个,差异表达的蛋白质参与的通路有Relaxin信号通路,心肌细胞中肾上腺素能信号通路和心肌收缩等;桥本甲状腺炎合并肥胖组与对照组进行对比,血清中差异蛋白质共有232个,差异蛋白质相关的通路有TRP通道的炎性反应介质调节,C型凝集素受体信号通路,Apelin信号通路;正常体质量的桥本甲状腺炎和桥本甲状腺炎合并肥胖组对比,结果显示血清中差异蛋白质共有212个,差异蛋白质富集的通路是雌激素信号通路。结论桥本甲状腺炎患者是否合并肥胖对血清中蛋白质表达具有一定的影响,合并肥胖时可能增加向甲状腺癌转化的风险。该结论为桥本甲状腺炎进一步的研究提供理论基础,对临床诊断与治疗具有重要意义。Objective To explore the effects of obesity and normal weight on serum proteomic differences in Hashimoto thyroiditis(HT)patients,and to observe the existing differential proteins and related pathways.Methods The serum samples from 10 obese and 10 normal weight patients with Hashimoto thyroiditis patients and from 10 controls were collected.The proteins in the serum were extracted and degraded by trypsin,analyzed by liquid chromatography-mass spectrometry,compared with the control group,identified the expression changes of differential proteins in the two groups,and analyzed by bioinformatics.Results There were 180 serum differential proteins were detected between Hashimoto thyroiditis group and control group;The differential proteins were involved in relaxin signal pathway,adrenergic signaling in cardiomyocytes and cardiac muscle contraction;There were 232 differential proteins in Hashimoto thyroiditis complicated with obesity group as compared with the control group.The differential proteins related to pathways were inflammatory mediators to regulate TRP channel,C-type lectin receptor signaling pathway and Apelin signaling pathway;The results showed that there were 212 differential proteins in serum between the Hashimoto thyroiditis patients with or without obesity group,and the pathway of differential protein enrichment was estrogen signaling pathway.Conclusions Both Hashimoto thyroiditis patients with or without obesity has a changed protein expression in serum but the change may increase the risk of developing thyroid cancer only in patients combined with obesity.The results provide a theoretical basis for further research of the disease and is of great significance for clinical diagnosis and treatment.

关 键 词：桥本甲状腺炎 肥胖 蛋白质组学 差异蛋白质 

分 类 号：R581[医药卫生—内分泌]