Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection  

作　　者：Jingwen LIU Jingjing GAO Enquan ZHANG Hanrui JIANG Guiling LI Jian LI Jun ZENG Daren WU 

机构地区：[1]College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China

出　　处：《Journal of Oceanology and Limnology》2023年第4期1547-1557,共11页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(Nos.42076086,41576166);the Natural Science Foundation of Fujian Province(No.2020J05138);the Education and Research Project for Young and Middle-aged Teachers of Fujian Province(No.JAT190343);the Cultivation Plan for Distinguished Young Scholars in Fujian Universities。

摘　　要：Lipidomics approach by UPLC-Q-Exactive-MS was used for the identification,quantification,comparison,and characterization of sphingolipids in virus infected marine Emiliania huxleyi BOF92 cells.The results show that 16 significantly changed sphingolipids(including Cer,CerG1,and SPHm)were identified during viral infection.Our data confirmed previously recognized facts that viral infection led to a shift toward virus-specific sphingolipids,which is consistent with the down-regulation of genes involved in the host de novo sphingolipid biosynthesis.Moreover,we revealed the upregulation of virusencoded homologous genes participating in de novo sphingolipids biosynthesis and virus-specific hydroxylated long chain bases(LCBs)as phytoCer,suggesting the competitive inhibition of host sphingolipid synthesis to produce the required building blocks for viral production,replication,and assembly.Additionally,Cer 40꞉1;2,Cer 40꞉2;2 isomer,and CerG139꞉0;2,Cer 39꞉0;2 as novel metabolite markers might indicate the general dysfunctions in E.huxleyi in response to viral infection.Our results show that viral infection led to a profound remodeling of host sphingolipidome,by which viruses depend on the hijacking of host sphingolipid metabolism to support the viral life cycle.

关 键 词：Emiliania huxleyi Coccolithovirus UPLC-Q-Exactive-MS sphingolipid metabolism biomarkers 

分 类 号：Q178[生物学—水生生物学]