甘蓝型油菜株高发育动态QTL分析  

QTL analysis of plant height development dynamics in Brassica napus L.

作　　者：何雨琦 余坤江 李远红 王倩 杨旭[1,2] 王显亚 田恩堂[1,2] HE Yu-qi;YU Kun-jiang;LI Yuan-hong;WANG Qian;YANG Xu;WANG Xian-ya;TIAN En-tang(Agricultural College of Guizhou University,Guiyang 550025,China;Oil Crops Research Institute of Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]贵州大学油料作物研究所,贵州贵阳550025

出　　处：《中国油料作物学报》2023年第4期684-693,共10页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(32060495);贵州省科技计划项目(黔科合基础[2020]1Y098);贵州大学培育项目(贵大培育[2019]47)。

摘　　要：矮化育种是解决油菜丰产、稳产、适宜机械化栽培重要途径之一。为进一步分析油菜株高调控的动态特征,寻找发育阶段的株高相关QTL,以一个由189个重组自交家系组成的甘蓝型油菜群体为材料,测定群体在2020年贵阳环境下5个生长阶段的株高净增长量和成熟期最终株高,分析表型变异和相关性,基于已构建的高密度分子标记遗传连锁图谱定位QTL,并在全基因组范围内鉴定阶段特异性表达的QTL,通过基因注释初步筛候选基因。结果表明,5个生长阶段群体的株高增长量均存在较大变异;株高增长量以前期(即进入抽薹期后的第一周)最大,随时间推移逐渐减小并趋于稳定;每个生长阶段株高净增长量都与前一个阶段呈显著正相关。在5个发育阶段共检测到60个QTL,包含4个主效QTL,8个QTL分别在两个不同生长阶段稳定表达,其余QTL只在某一个生长阶段特异性表达。在成熟期鉴定共到6个QTL,包含1个主效QTL。比较分析发现,成熟期的6个QTL在5个发育阶段并未检测到。本研究鉴定的5个主效QTL未曾报道。结合基因功能注释,初步筛选到15个株高相关候选基因。这些结果期待能有利于深化对株高发育的认识,为培育半矮秆油菜新品种提供依据。To achieve stable high yield and suitable mechanized cultivar for rapeseed(Brassica napus L.),QTLs on developmental plant height were studied using an population named AH,which composed of 189 recom-bined inbred members.Net increase in plant height of 5 growth stages and final plant height at maturity were investi-gated in Guiyang in 2020,and phenotypic variation and their correlations were analyzed.Based on previous con-structed high-density molecular marker genetic linkage map,QTL mapping was performed to identify the stage-spe-cific expression QTLs regulating plant height development on a genome-wide scale.Results showed great variations in plant height during the 5 growth stages after budding.Plant height changed the most in the 1st week,and the in-crease gradually slowed down over time.Net height growth at each stage was positively correlated with the previous stage.A total of 60 QTLs were detected in the 5 stages,including 4 major QTLs,8 QTLs stably expressed in 2 differ-ent stages,and the others(specifically expressed in at least one stage).6 QTLs were identified at maturity,including one major QTL.Comparative analysis showed that 6 QTLs at maturity stage were not detected at 5 developmental stages.Totally 5 major QTLs identified in this study have not been reported yet,thus can be used as new loci for de-velopmental plant height breeding in B.napus.Combined with gene function annotation,15 candidate genes related to plant height were preliminarily screened.It was expected to deepen the understanding of genetic regulation on rapeseed plant height,and provide new resources for semi-dwarf breeding.

关 键 词：甘蓝型油菜 株高 生长阶段 变异分析 相关性分析 QTL定位 

分 类 号：S565.4[农业科学—作物学]