芥菜型油菜NRAMP基因家族的鉴定与表达分析  

Genome-wide identification and expression analysis of NRAMP gene family in Brassica juncea

作　　者：黎婷 王家琪 霍卫聪 吴金锋 彭佳师[1] 周定港 严明理 张大为 LI Ting;WANG Jia-qi;HUO Wei-cong;WU Jin-feng;PENG Jia-shi;ZHOU Ding-gang;YAN Ming-li;ZHANG Da-wei(Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization,School of Life and Health Science,Hunan University of Science and Technology,Xiangtan 411201,China;Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of Chi-nese Academy of Agricultural Sciences,Wuhan 430062,China;Hunan Research Center of Heterosis Utilization in Rapeseed,Crop Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China)

机构地区：[1]经济作物遗传改良与综合利用湖南省重点实验室,湖南科技大学生命科学与健康学院,湖南湘潭411201 [2]农业农村部油料作物生物学与遗传育种重点实验室,中国农业科学院油料作物研究所,湖北武汉430062 [3]油菜杂种优势利用湖南省工程研究中心,湖南省农科院作物研究所,湖南长沙410125

出　　处：《中国油料作物学报》2023年第4期711-719,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(U19A2029,32071965);湖南省教育厅优秀青年项目(21B0490);农业农村部油料作物生物学与遗传育种重点实验室开放课题(KF2020011);湖南省大学生创新创业训练计划项目。

摘　　要：芥菜型油菜(Brassica juncea)对重金属有一定的耐受和积累能力,NRAMP(natural resistance associated macrophage protein,天然抗性相关巨噬细胞蛋白)蛋白参与金属离子的吸收和转运,在植物应对重金属胁迫响应中发挥重要作用。本研究以6个拟南芥NRAMP同源基因为参考,从芥菜型油菜中鉴定出18个BjNRAMP基因家族成员,分别定位在12条染色体上。系统进化分析结果表明BjNRAMP基因分属两个进化枝,其基因拷贝数目相比拟南芥增多可能是由于芥菜型油菜基因组三倍化所导致。对芥菜型油菜在不同浓度镉胁迫下根和叶的转录组测序数据进行分析发现,BjNRAMP基因的表达表现出组织特异性,其中BjNRAMP1.4基因在镉浓度为30 mg/kg胁迫下在根中被诱导表达;BjNRAMP2.2基因在镉浓度为10 mg/kg胁迫下在叶片中被诱导表达,而在根中的表达则受到抑制。克隆上述基因并转化至酵母,结果表明过表达BjNRAMP1.4可显著提高镉敏感型突变体酵母对镉的耐受性,而过表达BjNRAMP2.2提升作用有限,不及BjNRAMP1.4。本研究将为研究芥菜型油菜NRAMP基因家族成员结构以及功能提供一定的参考。Brassica juncea exhibites relatively tolerance to heavy metal cations.Because of NRAMP(natural resistance associated macrophage protein)is mainly involved in absorption and transport of metal cations,we used NRAMP homologous genes of Arabidopsis thaliana as reference,to better understand the NRAMPs in B.juncea.Thus 18 BjNRAMP memmbers in B.juncea were identified on 12 chromosomes.Phylogenetic analysis indicated that BjNRAMPs were clustered into 2 clades.Their expansions were possibly caused by B.juncea genome triplication af-ter the divergence of Arabidopsis and Brassica.Transcriptome sequencing data from roots and leaves under different concentration of cadmium(Cd)treatments indicated that expressions of BjNRAMPs were tissue-specific,in which BjNRAMP1.4 expression in root was increased under 30 mg/kg of Cd treatment.BjNRAMP2.2 expression in leaf was induced by 10 mg/kg of Cd treatment,but decreased in roots.After transformed into yeast,overexpression of BjN⁃RAMP1.4 in Cd-sensitive yeast mutants significantly improved its tolerance to Cd.Promotion function of BjN⁃RAMP2.2 played a limited role,not as good as BjNRAMP1.4.

关 键 词：芥菜型油菜 NRAMP基因家族 重金属 基因表达 

分 类 号：S565.4[农业科学—作物学]