根瘤菌Ⅲ型效应因子NopAA的突变及其对大豆共生结瘤的影响  

Mutation of NopAA in rhizobia and its effect on nodulation in soybean

作　　者：马胜男 张浩然 王悦 张煦杭 黄亚娸 王江旭 胡振帮[1] 辛大伟[1] 陈庆山[1] 王锦辉[1] MA Sheng-nan;ZHANG Hao-ran;WANG Yue;ZHANG Xu-hang;HUANG Ya-qi;WANG Jiang-xu;HU Zhen-bang;XIN Da-wei;CHEN Qing-shan;WANG Jin-hui(College of Agriculture,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院,黑龙江哈尔滨150086

出　　处：《中国油料作物学报》2023年第4期720-727,共8页Chinese Journal of Oil Crop Sciences

基　　金：中国博士后基金(2020M681072);国家自然科学基金(32072014,U20A2027)。

摘　　要：大豆根瘤固氮为大豆生长发育提供必不可少的氮源。根瘤菌Ⅲ型效应因子是大豆与根瘤菌共生体系建立过程中重要的信号分子。本研究以根瘤菌HH103 Ⅲ型效应因子NopAA为研究对象,通过生物信息学手段分析了其基本信息,利用三亲杂交构建NopAA突变体,并利用Southern blot对突变体进行鉴定。表达分析发现,NopAA突变显著降低了大豆免疫相关基因PR1的表达;在根瘤成熟之后,根瘤菌依旧能够表达NopAA。最后通过结瘤能力鉴定,发现NopAA突变能够显著抑制根瘤的产生。该研究为揭示根瘤菌Ⅲ型效应因子NopAA的作用机制奠定了基础,同时为大豆农业生产过程中共生固氮的利用提供理论基础。Soybean can symbiosis with rhizobia to produce nodule,through which symbiosis nitrogen fixation can provide essential nitrogen source for soybean growth and development.Rhizobia type III effectors are important signal molecules regulating symbiotic nitrogen fixation,which is very important for the establishment of symbiotic nitrogen fixation.In this study,NopAA mutants of rhizobia HH103 type III effecting factor were constructed and identified by bioinformatics analysis,three-parent hybridization and Southern blot were used to construct the mu-tant.Expression analysis showed that NopAA mutation significantly reduced the expression of soybean immune-re-lated gene PR1.Through NopAA expression detection during nodule formation,it was found that NopAA could still be expressed by rhizobia after nodule maturity.Finally,NopAA mutation can significantly inhibit the production of nodule through nodule formation ability identification.This study laid a foundation for revealing the mechanism of the type III effector NopAA and provided a theoretical basis for the utilization of co-organic nitrogen fixation in soy-bean agricultural production.

关 键 词：大豆 根瘤菌 Ⅲ型效应因子 表达分析 结瘤鉴定 

分 类 号：S565.1[农业科学—作物学]