基于TLR4/NF-κB信号通路探讨“气血并治”针法对神经根型颈椎病大鼠炎性损伤的影响  

Investigating the effect of“qi and blood combined treatment”acupuncture on inflammatory injury of cervical spondylotic radiculopathy rats based on TLR4/NF-κB signaling pathway

作　　者：王佳佳 嵇波[1] 刘翼天 石天宇 葛云鹏 谢亚娜 方洋 张玲[1] WANG Jiajia;JI Bo;LIU Yitian;SHI Tianyu;GE Yunpeng;XIE Yana;FANG Yang;ZHANG Ling(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京102488

出　　处：《中国医药导报》2023年第23期4-7,22,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(82174505)。

摘　　要：目的探讨“气血并治”针法对神经根型颈椎病(CSR)大鼠炎性损伤的治疗效应及与调节Toll样受体4(TLR4)/核因子κB(NF-κB)信号通路的相关性。方法24只5~7周龄(180~220 g)健康雄性SD大鼠,按照随机数字表法将其分为空白组、假手术组、模型组、“气血并治”针法组,每组6只。采用椎管插线法制备CSR模型,“气血并治”针法组于造模后第3天针刺双侧颈5和颈7夹脊穴、足三里和膈俞,颈夹脊穴予电针疏密波,2 Hz/50 Hz,强度1.5 mA,30 min/d,连续治疗14 d;空白组、假手术组、模型组束缚同“气血并治”针法组,不治疗。治疗结束后,比较各组步态评分,电镜观察脊髓各组超微结构,酶联免疫吸附试验法检测各组脊髓白细胞介素(IL)-1β和前列腺素E2(PGE2)含量,Western blot检测脊髓TLR4、NF-κB和环氧合酶2(COX-2)蛋白表达。结果治疗后,假手术组步态评分与空白组比较,差异无统计学意义(P>0.05),模型组步态评分较空白组升高(P<0.01),“气血并治”针法组步态评分较模型组降低(P<0.05)。空白组和假手术组大鼠髓鞘外形规则,结构完整;模型组髓鞘结构不规则,部分髓鞘断裂消失,厚薄不一,板层结构松散、扭曲、破裂,线粒体肿胀,甚至部分溶解消失;“气血并治”针法组髓鞘板层松散程度有所减轻,结构较紧凑,可见清晰的线粒体。假手术组IL-1β和PGE2蛋白表达水平与空白组比较,差异无统计学意义(P>0.05);模型组IL-1β和PGE2蛋白表达水平高于空白组(P<0.01);“气血并治”针法组IL-1β和PGE2蛋白表达水平低于模型组(P<0.01)。假手术组TLR4、NF-κB、COX-2蛋白相对表达量与空白组比较,差异无统计学意义(P>0.05);模型组TLR4、NF-κB、COX-2蛋白表达量较空白组升高(P<0.01);“气血并治”针法组TLR4、NF-κB、COX-2蛋白表达量低于模型组(P<0.01)。结论“气血并治”针法可以改善CSR大鼠的活动受限,其可能是通过阻抑TLR4/NF-κB信号通路Objective To investigate the therapeutic effect of qi and blood combined treatment acupuncture on inflammatory injury in rats with cervical spondylotic radiculopathy(CSR)and the correlation between toll-like receptor 4(TLR4)/nuclear factorκB(NF-κB)signaling pathway.Methods Twenty-four 5-7-week-old healthy male SD rats(180-220 g)were divided into blank group,sham surgery group,model group,and“qi and blood combined treatment”acupuncture group by random number table method,with six rats in each group.The CSR model was prepared by spinal canal insertion method.On the 3rd day after the model was made,the“qi and blood combined treatment”acupuncture group was treated by acupuncture at jiajǐpoints of cervical 5 and cervical 7,zusanlǐand géshù,and the cervical jia jǐpoints were treated by electro-acupuncture with density wave at 2 Hz/50 Hz,with an intensity of 1.5 mA for 30 min/d for 14 days.The restrain of blank group,sham operation group,and model group were the same as those of“qi and blood combined treatment”acupuncture group,no treatment.The restrain of the gait score of all groups was compared,the ultrastructure of the spinal cord was observed by electron microscope,the contents of interleukin(IL)-1βand prostaglandin E2(PGE2)in the spinal cord were detected by enzyme-linked immunosorbent assay,and the expression of TLR4,NF-κB,and cyclocycesterase 2(COX-2)in the spinal cord were detected by Western blot.Results After treatment,there was no significant difference in gait score between sham operation group and blank group(P>0.05),the gait score of model group was higher than that of blank group(P<0.01),and the gait score of“qi and blood combined treatment”acupuncture group was lower than that of model group(P<0.05).The appearance of myelin sheath of rats in blank group and sham operation group was regular and the structure was complete.In the model group,the myelin structure was irregular,some myelin was broken and disappeared,the thickness was different,the lamellar structure was loose,twisted

关 键 词：神经根型颈椎病 气血并治 炎症 Toll样受体4 核因子κ4 环氧合酶2 

分 类 号：R245[医药卫生—针灸推拿学]