膝三针对膝骨关节炎模型大鼠软骨形态学及行为学改变的影响  

Effect of three knee stitches on the morphological and behavioral changes of knee osteoarthritis model rat cartilage

作　　者：李仁重 孙奎 LI Renzhong;SUN Kui(The Second Clinical Medical College,Anhui University of Chinese Medicine,Anhui Province,Hefei 230061,China;Department of Orthopedics,the Second Affiliated Hospital of Anhui University of Chinese Medicine Anhui Acupuncture Hospital,Anhui Province,Hefei 230061,China)

机构地区：[1]安徽中医药大学第二临床医学院,安徽合肥230061 [2]安徽中医药大学第二附属医院安徽省针灸医院骨二科,安徽合肥230061

出　　处：《中国医药导报》2023年第24期16-19,47,共5页China Medical Herald

基　　金：安徽省财政厅、卫生健康委员会重大疾病治疗重点专科针灸科项目(皖财社〔2022〕622号);安徽省省级临床重点专科中医骨伤科项目(卫科教秘〔2017〕529号);安徽省教育厅重点项目(KJ2019A0472)。

摘　　要：目的探讨膝三针对膝骨关节炎(KOA)模型大鼠软骨形态学及行为学改变的影响。方法28只雄性SPF级SD大鼠(3~4周龄,体重180~220 g)采用随机数字表法分为空白组、模型组各8只,针刺组、西药组各6只。除空白组外均采用前交叉韧带横断术建立KOA大鼠模型。造模5周后,针刺组取左膝关节膝三针进行针刺治疗,1次/d,连续5 d休息2 d,治疗3周。西药组在患膝外搽双氯芬酸二乙胺乳胶剂。空白组和模型组不予干预。观察比较各组大鼠X线片、Lequesne MG评分、Mankin’s评分、软骨和HE染色后软骨形态学改变。结果与空白组比较,模型组大鼠左膝关节面间隙狭窄、关节面磨损及骨赘生成明显,关节软骨表层缺损明显,细胞数量减少,分布紊乱,Lequesne MG、Mankin’s评分升高(P<0.05);与模型组比较,针刺组治疗后Lequesne MG、Mankin’s评分降低(P<0.05),软骨表面微糙有光泽,细胞数量增多排列分明有序;模型组、针刺组、西药组治疗后Lequesne MG评分低于治疗前(P<0.05)。结论膝三针能改善KOA大鼠软骨形态学与行为学指标,治疗并保护其膝关节。Objective To investigate the effect of three knee stitches on the morphological and behavioral changes of knee osteoarthritis(KOA)model rats.Methods A total of 28 male SPF SD rats(three to four weeks old,weight 180 to 220 g)were divided into blank group and model group with eight rats,acupuncture group with six rats,and western medicine group with six rats respectively by random number table method.The rat model of KOA was established by anterior cruciate ligament transection except the blank group.After five weeks of modeling,the acupuncture group received three knee stitches from the left knee joint for acupuncture treatment,once a day,and rested for five consecutive days for two days for three weeks.In the western medicine group,Diclofenac Diethylamine Latex was applied outside the affected knee.Blank group and model group were not involved.The morphological changes of cartilage after X-ray film,Lequesne MG score,Mankin’s score,cartilage,and HE staining were observed and compared.Results Compared with blank group,the left knee articular surface space narrowed,articular surface wear,and osteophyte formation were obvious in model group,articular cartilage surface defect was obvious,the number and distribution of cells were decreased,and Lequesne MG and Mankin’s scores were increased(P<0.05).Compared with model group,Lequesne MG and Mankin’s scores in acupuncture group were decreased(P<0.05),the surface of cartilage was slightly rough and shiny,and the number of cells was increased and arranged in a clear and orderly manner.The Lequesne MG scores in model group,acupuncture group and western medicine group after treatment were lower than those before treatment(P<0.05).Conclusion Three knee stitches can improve the morphologic and behavioral indexes of the cartilage and protect the knee joint of KOA rats.

关 键 词：膝骨关节炎 膝三针 行为学 形态学 

分 类 号：R684[医药卫生—骨科学]