锦鲤疱疹病毒胸苷激酶基因的生物信息学分析  

Bioinformatics Analysis of Koi Herpesvirus Thymidine Kinase Gene

作　　者：陈莉 周广彪 郑耿东 温尔英 陈文婉 吴松浩 利光辉 CHEN Li;ZHOU Guang-biao;ZHENG Geng-dong(Shantou Customs District Technology Center,Shantou,Guangdong 515041;Shantou Customs Technology Center/Shantou University Science College Joint Laboratory of Aquatic Products,Shantou,Guangdong 515041)

机构地区：[1]汕头海关技术中心,广东汕头515041 [2]汕头海关技术中心/汕头大学理学院水产品联合实验室,广东汕头515041 [3]汕头海关动植处,广东汕头515057

出　　处：《安徽农业科学》2023年第16期90-94,共5页Journal of Anhui Agricultural Sciences

基　　金：汕头海关科研项目“广谱中和剂的研制及其对医用消毒用品微生物检验的影响”(2020STK009);汕头海关科研项目“观赏鱼类检疫性病毒生物信息学分析及病毒爆发的分子机制”(2022STK004);汕头海关科研项目“特殊样本核酸提取及测序分析在海关缉私鉴定中的应用研究”(2022STK005)。

摘　　要：[目的]探究锦鲤疱疹病毒胸苷激酶(Thymidine Kinase,TK)基因编码蛋白的生物学特性及结构功能特征。[方法]采用生物信息学软件对胸苷激酶蛋白的理化性质、亲/疏水性、结构域及二级结构和三级结构进行预测分析。[结果]TK蛋白编码224个氨基酸,不稳定系数较大,属于不稳定蛋白;且该蛋白不含有信号肽及跨膜结构,位于膜外;结构域位于2-175氨基酸残基区域,与其他病毒氨基酸序列比对分析,锦鲤疱疹病毒TK基因与鲤科疱疹病毒亲缘关系较近。[结论]对锦鲤疱疹病毒TK基因的分析,为深入研究锦鲤疱疹病毒的特性及分子机制,采取更有效的精准防控措施提供了基础保障,也为下一步的研究计划提供了方向。[Objective]To investigate the biological properties and structure⁃function characteristics of the protein encoded by thymidine kinase(TK)gene of koi herpesvirus.[Method]Bioinformatics software was used to predict the physicochemical properties,hydrophilicity/hydropho⁃bicity,domain and secondary structure and tertiary structure of the thymidine kinase protein.[Result]The results showed that TK protein en⁃codes 224 amino acids with a large instability coefficient,which is an unstable protein;and the protein does not contain signal peptide and transmembrane structure,which is located outside the membrane;the structural domain is located in the region of amino acid residues 2-175,and the analysis of amino acid sequence comparison with other viruses showed that the TK gene of koi herpesvirus is closely related to koi her⁃pesvirus.[Conclusion]The analysis of the TK gene of koi herpesvirus provides a basic guarantee for the in⁃depth study of the characteristics and molecular mechanism of koi herpesvirus and the adoption of more effective and precise prevention and control measures,and provides a direc⁃tion for the next research plan.

关 键 词：锦鲤疱疹病毒 胸苷激酶 生物信息学 

分 类 号：S917.4[农业科学—水产科学]