钙盐胁迫对百脉根种子萌发及苗期生长、生理生化的影响  

Effects of Calcium Salt Stress on Seed Germination and Seedling Growth,Physiological and Biochemical Characteristics of Lotus corniculatus

作　　者：赵文武 龚腾飞 赵丽丽[1] 谢文辉 ZHAO Wenwu;GONG Tengfei;ZHAO Lili;XIE Wenhui(College of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025

出　　处：《中国草地学报》2023年第8期70-78,共9页Chinese Journal of Grassland

基　　金：贵州省教育厅科技拔尖人才支持项目(黔教技[2022]076号);贵州省科技计划项目(黔科合平台人才[2021]5636号);国家自然科学基金(32060391、31560664)。

摘　　要：研究牧草对高钙盐的适应性在喀斯特地区山地草地的建植中具有十分重要的意义。以百脉根为试验材料,采用沙培法,设置5个钙盐梯度,分析钙盐胁迫下百脉根种子萌发、幼苗生长及相关生理指标的变化情况。研究结果表明:25 mmol/L CaCl2浓度下百脉根种子萌发及幼苗生长没有受到明显抑制,而随着钙盐浓度上升,百脉根种子发芽率降低、幼苗株高及生长速率下降,根长、根系分枝数和根尖数增加,叶片和根系中MDA含量增加,可溶性糖含量上升,SOD、POD活性先上升后下降;CAT活性在叶片中呈先上升后下降趋势,在根系中呈下降趋势;相关性分析表明根和叶对钙盐的响应不同,根系中各个指标与MDA的相关性都大于叶片。幼苗根系受钙盐胁迫影响较大,通过伸长根系、增加根系分枝和根尖数来吸收水分,以减轻高钙盐环境带来的伤害,同时通过增加渗透调节物质含量和增强酶活性等方式快速调节自身生理代谢功能,进而适应高钙胁迫环境,以上结果为百脉根抗盐新品种的培育及利用提供理论依据。It is crucial to assess the adaptability of forage grasses to high calcium salt concentrations for the planting of mountain pastures in karst areas.This study aimed to investigate the effects of calcium salt stress on seed germination and seedling growth,as well as physiological characteristics of Lotus corniculatus,five different calcium salt levels were set to simulate the conditions using sand culture method.The results showed that the seed germination and seedling growth remained unaffected at a CaCl2 concentration of 25 mmol/L.However,as the calcium salt concentration increased,the seed germination rate,and seedlings height,and growth rate declined.Conversely,root length,as well as the number of root branches and tips,exhibited an increased,accompanied by elevated levels of malondialdehyde(MDA)and soluble sugar in both leaves and roots.Furthermore,superoxide dismutase(SOD)and peroxidase(POD)activities initially increased and then decreased.The correlation analysis demonstrated distinct response of roots and leaves to calcium salt stress was different,with a stronger correlation between each index and MDA observed in roots than leaves.The root system of L.corniculatus seedlings responded to calcium salt stress by adapting its water absorption process through root system elongation and increasing the number of root branches and tips.These mechanisms aimed to mitigate the damage caused by high calcium and salt environment.Additionally,L.cornicula⁃tus seedlings rapidly regulated the physiological and metabolic functions by increasing the content of osmoregulatory substances and enhancing enzyme activities.These adaptive responses allowed the seedlings to cope with the high calcium stress environment.In conclusion,obtained results provided a theoretical basis for the cultivation and utilization of new salt-resistant varieties of L.corniculatus.

关 键 词：百脉根 钙盐胁迫 种子萌发 幼苗生长 生理 

分 类 号：S541.6[农业科学—作物学]