长毛对虾(Penaeus penicillatus)SNP标记开发及亲子鉴定技术研究  

DEVELOPMENT OF SNP MARKERS AND PARENTAGE ASSIGNMENT TECHNIQUES IN PENAEUS PENICILLATUS

作　　者：游淞元 杨乐 曾庆民[2] 张静[1] 黄良敏[1] 张东玲[1] 刘贤德[1] YOU Song-Yuan;YANG Le;ZENG Qing-Min;ZHANG Jing;HUANG Liang-Min;ZHANG Dong-Ling;LIU Xian-De(Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment,Fisheries College of Jimei University,Xiamen 361021,China;Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Fisheries Research Institute of Fujian,Xiamen 361013,China)

机构地区：[1]福建省海洋渔业资源与生态环境重点实验室集美大学水产学院,福建厦门361021 [2]福建省海洋生物增养殖与高值化利用重点实验室福建省水产研究所,福建厦门361013

出　　处：《海洋与湖沼》2023年第4期1165-1171,共7页Oceanologia Et Limnologia Sinica

基　　金：国家重点研发计划项目,2018YFD0901404号。

摘　　要：长毛对虾是我国南方沿海地区的重要经济虾类。近年来,由于过度捕捞、病害频发以及海洋环境恶化,长毛对虾的自然资源量大幅减少。为补充自然资源,改善种群结构,1980年我国开始对长毛对虾开展增殖放流工作。然而,增殖放流的效果如何,目前尚缺乏有效的评估手段。以长毛对虾为实验材料,通过对其基因组进行de novo测序和重测序开发SNP(single nucleotide polymorphism)标记,最终建立长毛对虾的亲子鉴定技术。具体研究结果如下:长毛对虾基因组大小为1335.76 Mb GC(guanine cytosine)含量为40.86%,N50为1221 bp;以组装的长毛对虾基因组为参考基因组通过重测序进行SNP挖掘,共获得12579780个原始SNP位点,经筛选,优化及模型选择,最终建立了基于192个SNP标记的长毛对虾亲子鉴定技术,亲子鉴定成功率为100%(95%的置信度)。研究结果可为下一步长毛对虾增殖放流效果评估提供参考。Penaeus penicillatus is an essential marine economic shrimp in the southeastern coastal regions of China.Wild populations of P.penicillatus have declined dramatically in recent years due to overfishing,marine environmental pollution,and other causes.Since 1980,China has initiated numerous stock-enhancing programs for P.penicillatus to replenish natural resources and improve population structure.However,no efficient evaluation method is available at present.SNP(single nucleotide polymorphism)markers were developed by De novo genome sequencing and resequencing in P.penicillatus,and parentage assignment techniques were established for P.penicillatus.Results show that the genome size of P.penicillatus is 1335.76 Mb,GC(guanine cytosine)content is 40.86%,and the assembly of the genome contigs N50 reaches 1221 bp.The whole-genome sequencing and SNP marker discovery were performed using the assembled P.penicillatus genome as the reference genome,yielding a total of 12579780 original SNP markers.Through screening,optimization,and model selection,a parentage assignment technique was developed for P.penicillatus based on 192 SNP markers,by which a parentage assignment rate of 100%with 95%confidence was achieved.This study offered a reference for future evaluation on the stock-enhancing effects for P.penicillatus.

关 键 词：长毛对虾 SNP 亲子鉴定 增殖放流 

分 类 号：Q953[生物学—动物学] Q789[农业科学—水产养殖] S968.2[农业科学—水产科学]