血浆Ghrelin/Obestatin系统对糖尿病大鼠早期胃排空的影响  

Effects of Plasma Ghrelin/Obestatin System on Early Gastric Emptying in Diabetic Rats

作　　者：卞丙凤 段滨红[1] 那威[1] 杜馥曼[1] 王旭萍[1] 仲恒[1] 向朝峰[1] BIAN Bingfeng;DUAN Binhong;NA Wei;DU Fuman;WANG Xuping;ZHONG Heng;XIANG Chaofeng(Department of Endocrinology,Heilongjiang Provincial Hospital,Harbin,Heilongjiang Province,150036 China)

机构地区：[1]黑龙江省医院内分泌科,黑龙江哈尔滨150036

出　　处：《糖尿病新世界》2023年第14期6-10,共5页Diabetes New World Magazine

基　　金：黑龙江省卫生健康委科研项目(2020-251)。

摘　　要：目的探讨糖尿病(diabetesmellitus,DM)大鼠早期血浆胃饥饿素(ghrelin)、肥胖抑制素(obestatin)水平及血浆Ghrelin/Obestatin系统与糖尿病胃排空紊乱的关系。方法于2020年11月—2022年6月,由黑龙江省医院内分泌科在哈尔滨医科大学实验动物医学部购买40只大鼠,将其随机分为正常对照组(NC组)10只、糖尿病组(DM组)10只、低剂量胰岛素组(LD组)10只、高剂量胰岛素组(HD组)10只。观察链脲佐菌素(strep⁃tozotocin,STZ)诱导的糖尿病大鼠早期液体胃排空率,以及血糖、血浆ghrelin和obestatin水平。结果造模2周后,DM组较NC组体质量下降、血糖增高,胃排空率明显加快,血浆ghrelin,obestatin及比值均增高,差异有统计学意义(P<0.05)。LD组较DM组体质量增加、血糖降低,胃排空率低于DM组,血浆obestatin较NC组高,低于DM,Ghrelin/Obestatin比值较DM组降低,差异有统计学意义(P<0.05)。HD组较LD组体质量增加、血糖降低,胃排空率低于LD组,血浆obestatin较LD组低、较NC组高,低于DM组,Ghrelin/Obestatin比值较LD组降低,差异有统计学意义(P<0.05)。造模6周后,DM组较NC组体质量下降、血糖增高,胃排空率明显加快,血浆ghrelin,obestatin及比值均增高,差异有统计学意义(P<0.05)。LD组较DM组体质量增加、血糖降低,胃排空率较DM组减慢,血浆obestatin较NC组增加,低于DM组,Ghrelin/Obestatin比值较DM组降低,差异有统计学意义(P<0.05)。HD组较LD组体质量增加、血糖降低,胃排空率较LD组减慢,血浆obestatin较LD组低、较NC组增加,低于DM组,Ghrelin/Obestatin比值较LD组降低,差异有统计学意义(P<0.05)。结论糖尿病早期大鼠存在胃排空加快现象。血浆ghrelin与obestatin水平均显著增高,两者比值亦升高,故Ghrelin/Obestatin升高,可能是其胃排空加快的原因之一。外源性补充中效胰岛素后,对血浆obestatin水平的影响可能与调节胃排空有一定关系。Objective To investigate the relationship between plasma Ghrelin and Obestatin levels,plasma Ghrelin/Obestatin system and diabetic gastric emptying disorder in the early stage of Diabetesmellitus(DM)rats.Methods From November 2020 to June 2022,40 rats from the Department of Experimental Animal Medicine of Harbin Medical University were purchased by the Department of Endocrinology of Heilongjiang Provincial Hospital and randomly di⁃vided into 10 normal control group(NC group),10 diabetic group(DM group),10 low-dose insulin group(LD group)and 10 high-dose insulin group(HD group).The early liquid gastric emptying rate,blood glucose,plasma ghrelin and obestatin levels of streptozotocin(STZ)induced diabetic rats were observed.Results After 2 weeks of modeling,com⁃pared with NC group,the body mass and blood glucose in DM group decreased,the gastric empting rate was signifi⁃cantly accelerated,and plasma ghrelin,obestatin and ratio were increased,the difference was statistically significant(P<0.05).Compared with DM group,LD group body mass increased,blood glucose decreased,gastric empting rate was lower than DM group,plasma obestatin was higher than NC group but lower than DM group,and Ghrelin/Obestatin ra⁃tio was lower than DM group,the difference was statistically significant(P<0.05).Compared with LD group,the body mass and blood glucose of HD group increased,gastric empting rate was lower than LD group,plasma obestatin was lower than LD group,higher than NC group,lower than DM group,Ghrelin/Obestatin ratio was lower than LD group,the difference was statistically significant(P<0.05).After 6 weeks of modeling,compared with NC group,the body mass and blood glucose in DM group decreased,the gastric empting rate was significantly accelerated,and plasma ghrelin,obestatin and ratio were increased,the difference was statistically significant(P<0.05).Compared with the DM group,the body mass and blood glucose of LD group increased,the gastric empting rate slowed down,the plasma obestatin increased compared with the NC g

关 键 词：胃饥饿素 肥胖抑制素 胃饥饿素/肥胖抑制素 胃排空紊乱 

分 类 号：R587.1[医药卫生—内分泌]