微流控芯片上单细胞操控与分析方法研究进展  

Recent advances in single-cell manipulation and analysis methods on microfluidic chips

作　　者：宋扬[1] 林金明[1] Yang Song;Jin-Ming Lin(Department of Chemistry,Beijing Key Laboratory of Microanalytical Methods and Instrumentation,Tsinghua University,Beijing 100084,China)

机构地区：[1]清华大学化学系,微量分析测试与仪器研制北京市重点实验室,北京100084

出　　处：《中国科学：化学》2023年第8期1472-1493,共22页SCIENTIA SINICA Chimica

基　　金：国家重点研发计划(No.2022YFC3400700);国家自然科学基金项目(No.22034005)资助。

摘　　要：单细胞水平的异质性已经被人们广泛认识,而传统的细胞分析会忽视这种异质性,限制了细胞生物学、医学等领域的发展.为了深入研究细胞异质性,需要在单细胞水平上对细胞进行多样化的操作和全面的分析.由于在微通道内对微纳升尺度流体的精确控制能力,以及高通量、自动化、低污染等优点,微流控技术成为单细胞分析的理想技术.本文综述了近年微流控技术在单细胞操控与分析中的应用,总结了一些有前景的微流控单细胞操控方法的原理和研究进展,包括流体动力学、电学、声学、光学和磁学方法,以及与微流控芯片联用的荧光、电化学、质谱和拉曼光谱检测方法.最后,我们还讨论了微流控芯片上单细胞操控与分析的研究趋势.Cell heterogeneity has been widely recognized at the single-cell level.Traditional cell analysis ignores this heterogeneity,which limits the development of cell biology,medicine and other fields.To study cell heterogeneity in depth,diverse manipulations and comprehensive analyses of cells at the single cell level are required.Microfluidics is an ideal technology for single cell analysis due to the ability to precisely control micro/nanoliter fluids within the microchannel,as well as the advantages of high throughput,automation,and low contamination.In this paper,we review the recent applications of microfluidics in single-cell manipulation and analysis.The principles and research progress of some promising microfluidic single-cell manipulation methods are summarized,including hydrodynamic,electrical,acoustic,optical and magnetic methods.Fluorescence,electrochemical,mass spectrometry and Raman spectrometry detection methods coupled with microfluidic chips are also described.Finally,we discuss the research trends in single-cell manipulation and analysis on microfluidic chips.

关 键 词：微流控 单细胞操控 单细胞分析 

分 类 号：TN492[电子电信—微电子学与固体电子学] Q2-33[生物学—细胞生物学]