氯硝柳胺抑制宫颈癌细胞增殖和迁移  

Niclosamide Inhibits the Proliferation and Migration of Cervical Cancer Cells

作　　者：罗艳林 陈义兵[3] LUO Yanlin;CHEN Yibing(Department of Gynecologic Oncology,the Affiliated Cancer Hospital of Zhengzhou University/Henan Cancer Hospital,Zhengzhou 450008,China;Institute of Clinical Pharmacology,School of Basic Medical Science,Academy of Medical Science,Zhengzhou University,Zhengzhou 450001,China;Department of Gynecology and Obstetrics,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学附属肿瘤医院/河南省肿瘤医院妇科,河南郑州450008 [2]郑州大学基础医学院临床药理研究所,河南郑州450001 [3]郑州大学第一附属医院妇产科,河南郑州450052

出　　处：《河南医学研究》2023年第16期2891-2895,共5页Henan Medical Research

基　　金：河南省卫生科技攻关计划省部共建重点项目(SBGJ2020002081)。

摘　　要：目的研究氯硝柳胺对宫颈癌细胞系HeLa和SiHa细胞增殖、迁移的影响,并初步探讨其可能的分子机制。方法分别采用MTT法、细胞划痕试验检测氯硝柳胺对宫颈癌细胞系HeLa和SiHa细胞增殖、迁移的影响,并通过Western blot方法检测氯硝柳胺对HeLa和SiHa细胞肿瘤转移相关基因Vimentin、N-Cadherin蛋白表达的影响。结果氯硝柳胺以浓度依赖性和时间依赖性的方式抑制宫颈癌细胞系HeLa和SiHa细胞增殖(P<0.05)。4μmol·L^(-1)的氯硝柳胺作用HeLa细胞48、72 h后,细胞活力分别是(24.15±1.13)%和(16.22±2.63)%,作用SiHa细胞48、72 h后,细胞活力分别是(37.23±1.52)%和(23.18%±2.82)%。在0.5μmol·L^(-1)浓度时,氯硝柳胺对细胞增殖的抑制差异无统计学意义(P>0.05),但能抑制细胞的迁移(P<0.05)。另外,0.5~4μmol·L^(-1)的氯硝柳胺均可抑制Vimentin、N-Cadherin蛋白的表达,可能是通过抑制细胞上皮间质转化(EMT)行使对宫颈癌细胞的抗肿瘤作用。结论氯硝柳胺以剂量依赖性和时间依赖性的方式降低宫颈癌细胞活力,抑制宫颈癌细胞转移,促进宫颈癌细胞凋亡,抑制Vimentin和N-Cadherin蛋白表达,可能成为一种新的治疗宫颈癌的药物。Objective To study the effect of niclosamide on the proliferation,metastasis inhibition of cervical cancer cell lines,HeLa and SiHa,and to explore its possible molecular mechanism.Methods MTT assay,cell wound healing assay were used to detect the effects of niclosamide on the cell proliferation and migration of cervical cancer cell lines,HeLa and SiHa,respectively.And the effects of niclosamide on the expression of Vimentin and N-Cadherin proteins related to tumor metastasis in HeLa and SiHa cells were detected by Western blot.Results Niclosamide inhibited the proliferation of cervical cancer cell lines,HeLa and SiHa,in a dose dependent and time dependent manner(P<0.05).After 48 and 72 hours of treatment with niclosamide at 4μmol·L^(-1),the cell viability of HeLa was(24.15±1.13)%and(16.22±2.63)%,respectively,and SiHa cells was(37.23±1.52)%and(23.18%±2.82)%,respectively.At a concentration of 0.5μmol·L^(-1),there was no statistically significant difference in the inhibitory effect of niclosamide on cell proliferation(P>0.05),but could inhibit cell migration(P<0.05).Additionally,the expression of Vimentin and N-Cadherin protein was inhibited at 0.5μmol·L^(-1) to 4μmol·L^(-1) concentrations,which may be attributed to the anti-tumor effect on cervical cancer cells by inhibiting epithelial mesenchymal transformation(EMT).Conclusion Niclosamide can reduce the viability of cervical cancer cells in a dose dependent manner and time dependent manner,inhibit the metastasis of cervical cancer cells,promote the apoptosis of cervical cancer cells,inhibit the expression of Vimentin and N-Cadherin proteins,and may be a new drug for cervical cancer.It suggests that niclosamide might be a new drug for the treatment of cervical cancer.

关 键 词：氯硝柳胺 宫颈癌 增殖 迁移 上皮间质转化 

分 类 号：R711.74[医药卫生—妇产科学]