LecRKIII.2基因调控拟南芥对非生物胁迫和外源激素的响应  

LecRKIII.2 Gene Regulates Response of Arabidopsis thaliana to Abiotic Stress and Exogenous Hormones

作　　者：谢道龙 邹肖肖 李美玲 游昌乔 周苹[1,2] 肖文君 郭新红[1,2] XIE Daolong;ZOU Xiaoxiao;LI Meiling;YOU Changqiao;ZHOU Ping;XIAO Wenjun;GUO Xinhong(Chongqing Research Institute,Hunan University,Chongqing 400039,China;Hunan Provincial Key Laboratory of Plant Functional Genomics and Developmental Regulation(Hunan University),Changsha Hunan 410082,China)

机构地区：[1]湖南大学重庆研究院,重庆400039 [2]植物功能基因组学与发育调控湖南省重点实验室(湖南大学),湖南长沙410082

出　　处：《广西师范大学学报（自然科学版）》2023年第4期189-199,共11页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(31872866);重庆市自然科学基金(2022NSCQ-MSX5788,2022NSCQ-MSX5762);长沙市自然科学基金(kq2202149);湖南省自然科学青年基金(2022JJ40051)。

摘　　要：在拟南芥Arabidopsis thaliana中,凝集素类受体激酶(lectin receptor-like kinases,LecRKs)包含86个成员,在植物对生物及非生物胁迫反应中起关键作用。AtLecRKIII.2作为LecRKs家族的一员,其具体功能未见报道。本文通过实时定量PCR和三引物法鉴别AtLecRKIII.2基因的过表达植株及纯合缺失突变体。启动子顺式作用元件分析发现,AtLecRKIII.2基因有多种与调控胁迫、激素等反应相关的功能元件。组织表达模式分析表明,AtLecRKIII.2基因于果荚中具有较高的转录水平,茎中的转录水平次之。逆境及激素响应模式分析表明,AtLecRKIII.2基因对外源生长素(3-indoleacetic acid,IAA)、油菜素内酯(brassinolide,BL)、赤霉素(gibberellins,GAs)、PEG8000、高温和低温均具有不同程度的响应。萌发率及根长实验结果表明,NaCl或Mannitol处理后,LecRKIII.2-OE的种子萌发率高于野生型,而lecrkiii.2的种子萌发率低于野生型。用生长素处理后,LecRKIII.2-OE根的伸长被显著抑制,且其体内与生长素应答和运输相关基因的转录水平显著变化。上述研究结果表明,AtLecRKIII.2基因正调控拟南芥对盐胁迫、渗透胁迫的耐受性和涉及根的生长发育。研究结果为进一步深入研究AtLecRKIII.2基因在植物非生物胁迫及激素信号转导中的生理功能提供参考数据。In Arabidopsis thaliana,lectin receptor-like kinases(LecRKs)contain 86 members and are widely involved in plant resistance to biotic and abiotic stresses.AtLecRKIII.2 is a member of the LecRKs family and its specific function has not been reported.Here,three-primer method and real-time quantitative PCR are used to identify homozygous deletion mutants and overexpression plants of AtLecRKIII.2 gene.Analysis of promoter cis-acting elements reveals that AtLecRKIII.2 gene has multiple regulatory elements related to stress,hormone and other responses.Tissue expression pattern analysis shows that AtLecRKIII.2 gene has the highest expression in fruit pods,followed by stems.The analysis of adversity and hormone response patterns shows that AtLecRKIII.2 gene has different degrees of response to exogenous hormones(IAA,BL,GA),PEG8000,high temperature and low temperature.The experimental results of germination rate and root length shows that after treated with NaCl or Mannitol,the seed germination rate of LecRKIII.2-OE is higher than that of the wild type,while lecrkiii.2 is lower than that of the wild type.After treated with auxin,the elongation of LecRKIII.2-OE roots is significantly inhibited and the expression level of auxin-related genes in LecRKIII.2-OE is significantly changed.The above research results indicate that the AtLecRKIII.2 gene may positively regulate the tolerance of Arabidopsis to salt stress and osmotic stress and the growth and development of roots.The above results provide reference data for further research of the physiological function of AtLecRKIII.2 gene in plant abiotic stress and hormone signal transduction.

关 键 词：拟南芥 凝集素类受体激酶 AtLecRKIII.2 非生物胁迫 激素 

分 类 号：Q943.3[生物学—植物学]