雷公藤内生细菌两两混合培养对促生物质生产的影响  

Effect of Dual Mixed Culture of Endophytic Bacteria from Tripterygium wilfordii on Production of Plant Growth Promoting Substances

作　　者：孙睿[1] 宋萍[1,2] 刘洁 洪伟[1,2] 吴承祯[2,3] 封磊[4] SUN Rui;SONG Ping;LIU Jie;HONG Wei;WU Chengzhen;FENG Lei(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China;Fujian Provincial Key Laboratory of Forest Ecosystem Processing and Management(Fujian Agriculture and Forestry University),Fuzhou Fujian 350002,China;Wuyi University,Wuyishan Fujian 354300,China;College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建省高校森林生态系统过程与经营重点实验室(福建农林大学),福建福州350002 [3]武夷学院,福建武夷山354300 [4]福建农林大学资源与环境学院,福建福州350002

出　　处：《广西师范大学学报（自然科学版）》2023年第4期220-230,共11页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(31000264,31470577);福建省自然科学基金(2022J01124);福建农林大学科技创新专项基金(CXZX2016061,CXZX2018133)。

摘　　要：以分离自药用植物雷公藤Tripterygium wilfordii的23株内生细菌为材料,测定各菌株在单独培养及两两混合(253个组合)培养条件下产IAA、ACC脱氨酶、铁载体和溶解磷酸盐的能力,并研究内生细菌共存互作对菌株促生物质生产的影响。结果表明:菌株共培养产生的相互作用使各种促生物质生产发生明显改变。与单独培养相比,混合培养情况下,大多数菌株的溶磷能力和IAA分泌量有较大提升,与菌株LY4混合培养的菌株其溶磷量具有更大的提高,而菌株LG3、LJ3、LJ12的混合菌株具有更高的IAA分泌量。菌株LJ12的混合菌株具有较高的ACC脱氨酶活性,菌株LJ12、LY3对其混合菌株的铁载体合成具有重要贡献。相关性分析显示,大部分菌株的混合菌株与单菌株的各促生指标并没有明显的相关性,证明在菌株共存互作下,混合菌株的促生性能并非取决于单菌株的促生能力。In this study,23 endophytic bacteria isolated from the medicinal plant Tripterygium wilfordii were applied as experimental strains to determine the production of IAA,ACC deaminase and siderophore and phosphate solubilization capacity in the monoculture and dual mixed culture(253 combinations).By comparison,the effects of coexistence and interactions of endophytic bacteria on growth-promoting substance production were evaluated.The results indicated that interactions between co-cultured bacteria obviously changed the production of various growth-promoting substances.Compared with the monoculture of strains,abilities of phosphate solubilization and IAA secretion in most of the mixed culture had great improvement.The strains that were co-cultured with LY4 were found to have larger increment of phosphate solubilization.The strain mixtures including LG3,LJ3 or LJ12 showed higher secretion of IAA.Higher ACC deaminase activity were tested from the strain mixtures including LJ12.The strains LJ12 and LY3 had important contribution to siderophore production of their strain mixtures.The growth-promoting index of the mixtures of most of the strains showed no significant correlation to those of the single strains.The growth-promoting properties of the mixtures of the endophytic bacteria did not depend on the abilities of the single strains.

关 键 词：雷公藤 内生细菌 混合培养 促生 

分 类 号：S567.19[农业科学—中草药栽培] Q939.96[农业科学—作物学]