盐穗木HcALDH7B4基因的原核表达增强耐盐抗旱性  

Prokaryotic expression of HcALDH7B4 gene enhanced salt tolerance and drought resistance

作　　者：毛晓菲 黄世平 银芳柳 曾幼玲 MAO Xiaofei;HUANG Shiping;YIN Fangliu;ZENG Youling(Xinjiang Key Laboratory Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830000,China)

机构地区：[1]新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐830000

出　　处：《新疆农业科学》2023年第8期2006-2012,共7页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(31760071)。

摘　　要：【目的】研究盐穗木乙醛脱氢酶7B4基因(HcALDH7B4)在原核细胞中的耐盐抗旱功能,为耐盐抗旱育种提供候选基因。【方法】利用qRT-PCR检测高盐和干旱胁迫下HcALDH7B4基因的表达水平;利用原核表达重组大肠杆菌(pET-28a-HcALDH7B4)在盐旱胁迫下的菌落大小和生长活力分析其盐旱抗性。【结果】在600 mM NaCl处理和自然干旱条件下,盐穗木同化枝中HcALDH7B4被显著诱导。相较对照菌株(pET-28a),原核表达的重组大肠杆菌在500 mM NaCl、500 mM KCl和500 mM、800 mM Mannitol胁迫下表现出更好的生长活力。【结论】盐穗木HcALDH7B4是一个胁迫应答基因,可以提高重组菌盐旱胁迫的耐受性。【Objective】To study the salt tolerance and drought resistance function of Halostachys caspica aldehyde dehydrogenase gene(HcALDH7B4)in prokaryotic cells in the hope of providing possible candidate genes for salt tolerance and drought resistance breeding.【Methods】qRT-PCR was used to detect the expression level of HcALDH7B4 gene under high salt and drought stress.The colony size and growth activity of prokaryotic expression recombinant E.coli(PET-28A-HCALDH7B4)under salt and drought stress were used to analyze its salt and drought resistance.【Results】The results of qRT-PCR showed that HcALDH7B4 was significantly induced in the assimilated branches under 600 mmol/L NaCl treatment and natural drought condition and compared with the control strain(PET-28A),the recombinant E.coli expressed by prokaryotic expression showed better growth activity under 500 mmol/L NaCl,500 mmol/L KCl and 500 mmol/L and 800 mmol/L Mannitol stress.【Conclusion】HcALDH7B4 is a stress response gene,and can improve the tolerance of recombinant strains to salt and drought stress.

关 键 词：盐穗木 HcALDH7B4 原核表达 耐盐耐旱 

分 类 号：S188[农业科学—农业基础科学]