石榴皮瓤提取物对成年小鼠体重增长及血浆免疫相关指标的影响  

Effect of pomegranate peel flesh extract on body weight gain and plasma Immunity-related indexes in adult mice

作　　者：刘佳佳 韩涛[2] LIU Jiajia;HAN Tao(Institute of Animal Husbandry,Xinjiang Academy of Animal Sciences,Urumqi 830011,China;Institute of Veterinary Medicine,Xinjiang Academy of Animal Sciences,Urumqi 830011,China)

机构地区：[1]新疆畜牧科学院畜牧研究所,乌鲁木齐830011 [2]新疆畜牧科学院兽医研究所,乌鲁木齐830011

出　　处：《新疆农业科学》2023年第8期2074-2080,共7页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重点研发计划项目“肉鸽产业提质增效关键技术集成与示范”(2020B01007-2)。

摘　　要：【目的】研究石榴皮瓤提取物对小鼠体重、血浆抗氧化和促炎因子水平的影响。【方法】选择平均体重(37.31±5.57)g的成年小鼠30只,随机分为2组,每组15只,分别为对照组和试验组。在相同基础日粮条件下试验组小鼠灌喂1.5 g/(d·只)石榴皮瓤粗提物[即鞣花酸的灌喂量为40.74 mg/(d·只)],对照组小鼠灌喂相应体积的无菌蒸馏水,灌喂试验21 d。试验结束当天,小鼠晨饲前空腹称量体重,断尾采集小鼠血液。【结果】(1)试验组小鼠的末重、总增重和平均日增重分别比对照组显著提高了8.21%(P<0.05)、47.56%(P<0.05)和48.39%(P<0.05);(2)试验组小鼠血浆中SOD、CAT、和T-AOC水平分别比对照组显著提高了24.44%(P<0.05)、54.82%(P<0.05)和27.69%(P<0.05),抑制羟自由基能力和抗超氧阴离子活力分别比对照组极显著提高了36.01%(P<0.01)和43.21%(P<0.01),显著降低了试验组小鼠血浆中MAD的浓度(P<0.05);(3)试验组小鼠血浆中TNF-α和IL-17分别比对照组显著降低了17.16%(P<0.05)和30.34%(P<0.05)。【结论】对小鼠灌喂石榴皮瓤提取物可显著增加小鼠体重,提高小鼠抗氧化能力和抗炎症能力。【Objective】This experiment aims to investigate the effects of pomegranate peel flesh extract on body weight,plasma antioxidant and pro-inflammatory factor levels in mice in the hope of providing a theoretical basis for further research on the biological effects of pomegranate peel flesh extract.【Methods】Thirty adult male mice with an average body weight of(37.31±5.57)g were randomly divided into 2 groups:the control group and the test group,with 5 replicates of 3 mice in each group,for a total of 15 mice.Mice in the test group were fed 1.5 g/(d-animal)of crude extract of pomegranate peel flesh[i.e.,ellagic acid at 40.74 mg/(d-animal)]under the same basal diet,and mice in the control group were fed the corresponding quantity of sterile distilled water for 21 days.At the end of the experiment,mice were weighed on an empty stomach before morning feeding,and blood was collected from the mice with their tails severed.【Results】The results showed that(1)the end weight,total weight gain and mean daily weight gain of mice in the test group were significantly increased by 8.21%(P<0.05),47.56%(P<0.05)and 48.39%(P<0.05),respectively,compared with the control group;(2)the plasma levels of SOD,CAT,and T-AOC in mice in the test group were significantly increased by 24.44%(P<0.05),54.82%(P<0.05),and 27.69%(P<0.05),respectively,and the ability to inhibit hydroxyl radicals and anti-superoxide anion activity were highly significantly increased by 36.01%(P<0.01)and 43.21%(P<0.01),respectively,compared with the control group,which significantly reduced the concentration of MAD in the plasma of mice in the test group(P<0.05);(3)the plasma levels of TNF mice plasma TNF-αand IL-17 were significantly reduced by 17.16%(P<0.05)and 30.34%(P<0.05),respectively,compared with the control group.【Conclusion】In conclusion,under the present experimental conditions,the administration of pomegranate peel flesh extract to mice significantly increases their body weight and improves their antioxidant capacity and anti-inflammatory abi

关 键 词：石榴皮瓤提取物 小鼠 体重 抗氧化 炎症 

分 类 号：S852[农业科学—基础兽医学]