Identification of stable quantitative trait loci underlying waterlogging tolerance post-anthesis in common wheat(Triticum aestivum)  

作　　者：Fugong Ding Jingyang Tong Rui Xu Jing Chen Xiaoting Xu Muhammad Nadeem Shuping Wang Yingxin Zhang Zhanwang Zhu Fengju Wang Zhengwu Fang Yuanfeng Hao 

机构地区：[1]MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River(Co-construction by Ministry and Province),College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China [2]National Wheat Improvement Centre,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Queensland Alliance for Agriculture and Food Innovation,The University of Queensland,St Lucia,QLD 4072,Australia [4]Food Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China

出　　处：《The Crop Journal》2023年第4期1163-1170,共8页作物学报（英文版）

基　　金：Chinese Scholarship Council for financial support (202203250009);financially supported by the Key Research and Development Program of Hubei Province (2021BBA225);the Agricultural Science and Technology Innovation Program;the Fundamental Research Funds for Central Non-Profit of the Institute of Crop Sciences, CAAS。

摘　　要：Waterlogging is a growing threat to wheat production in high-rainfall areas.In this study,a doubled haploid(DH) population developed from a cross between Yangmai 16(waterlogging-tolerant) and Zhongmai895(waterlogging-sensitive) was used to map quantitative trait loci(QTL) for waterlogging tolerance using a high-density 660K single-nucleotide polymorphism(SNP) array.Two experimental designs,waterlogging concrete tank(CT) and waterlogging plastic tank(PT),were used to simulate waterlogging during anthesis in five environments across three growing seasons.Waterlogging significantly decreased thousand-kernel weight(TKW) relative to non-waterlogged controls,although the degree varied across lines.Three QTL for waterlogging tolerance were identified on chromosomes 4AL,5AS,and 7DL in at least two environments.All favorable alleles were contributed by the waterlogging-tolerant parent Yangmai16.QWTC.caas-4AL exhibited pleiotropic effects on both enhancing waterlogging tolerance and decreasing plant height.Six high-confidence genes were annotated within the QTL interval.The combined effects of QWTC.caas-4AL and QWTC.caas-5AS greatly improved waterlogging tolerance,while the combined effects of all three identified QTL(QWTC.caas-4AL,QWTC.caas-5AS,and QWTC.caas-7DL) exhibited the most significant effect on waterlogging tolerance.Breeder-friendly kompetitive allele-specific PCR(KASP) markers(K_AX_111523809,K_AX_108971224,and K_AX_110553316) flanking the interval of QWTC.caas-4AL,QWTC.caas-5AS,and QWTC.caas-7DL were produced.These markers were tested in a collection of 240 wheat accessions,and three superior polymorphisms of the markers distributed over 67elite cultivars in the test population,from the Chinese provinces of Jiangsu,Anhui,and Hubei.The three KASP markers could be used for marker-assisted selection(MAS) to improve waterlogging tolerance in wheat.

关 键 词：Genetic mapping QTL Waterlogging tolerance WHEAT 

分 类 号：S512.1[农业科学—作物学]