Identification of Green-Revertible Yellow 3(GRY3),encoding a 4-hydroxy-3-methylbut-2-enyl diphosphate reductase involved in chlorophyll synthesis under high temperature and high light in rice  

作　　者：Hongzhen Jiang Anpeng Zhang Banpu Ruan Haitao Hu Rui Guo Jingguang Chen Qian Qian Zhenyu Gao 

机构地区：[1]China National Rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310006,Zhejiang,China [2]School of Agriculture,Shenzhen Campus of Sun Yat-sen University,Shenzhen 518107,Guangdong,China [3]Institute of Crop Breeding and Cultivation,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China [4]College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,Zhejiang,China

出　　处：《The Crop Journal》2023年第4期1171-1180,共10页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (32188102 and 31901481)。

摘　　要：Chlorophyll,a green pigment in photosynthetic organisms,is generated by two distinct biochemical pathways,the tetrapyrrole biosynthetic pathway(TBP) and the methylerythritol 4-phosphate(MEP)pathway.MEP is one of the pathways for isoprenoid synthesis in plants,with 4-hydroxy-3-methylbut-2-enyl diphosphate reductase(HDR) catalyzing its last step.In this study,we isolated a greenrevertible yellow leaf mutant gry3 in rice and cloned the GRY3 gene,which encodes a HDR participating in geranylgeranyl diphosphate(GGPP) biosynthesis in chloroplast.A complementation experiment confirmed that a missense mutation(C to T) in the fourth exon of LOC_Os03g52170 causes the gry3 phenotype.Under high temperature and high light,transcript and protein abundances of GRY3 were reduced in the gry3 mutant.Transcriptional expression of chlorophyll biosynthesis,chloroplast development,and genes involved in photosynthesis were also affected.Excessive reactive oxygen species accumulation,cell death,and photosynthetic proteins degradation were occurred in the mutant.The content of GGPP was reduced in gry3 compared with Nipponbare,resulting in a stoichiometric imbalance of tetrapyrrolic chlorophyll precursors.These results shed light on the response of chloroplast biogenesis and maintenance in plants to high-temperature and high-light stress.

关 键 词：CHLOROPLAST High temperature High light RICE 

分 类 号：S511[农业科学—作物学]