A cluster of mutagenesis revealed an osmotic regulatory role of the OsPIP1 genes in enhancing rice salt tolerance  

作　　者：Leyuan Tao Bing Wang Shichao Xin Wei Li Shengcai Huang Laihua Liu Jing Cui Qianru Zhang Xianguo Cheng 

机构地区：[1]State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Department of Plant Nutrition,College of Resources and Environmental Sciences,China Agricultural University,Beijing 100193,China [3]Key Laboratory of Rubber Biology and Genetic Resources of the Ministry of Agriculture,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571011,Hainan,China [4]International Joint Research Center of Technology Innovation and Achievement Transformation on Palatability of Rice,Tianjin Agricultural University,Tianjin 300384,China

出　　处：《The Crop Journal》2023年第4期1204-1217,共14页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (2021YFF1000402-2);the Key Project of Transgenic Crops Cultivation (2016ZX08010005-9)。

摘　　要：Aquaporins play important regulatory roles in improving plant abiotic stress tolerance.To better understand whether the Os PIP1 genes collectively dominate the osmotic regulation in rice under salt stress,a cluster editing of the Os PIP1;1,Os PIP1;2 and Os PIP1;3 genes in rice was performed by CRISPR/Cas9 system.Sequencing showed that two mutants with Cas9-free,line 14 and line 18 were successfully edited.Briefly,line 14 deleted a single C base in both the Os PIP1;1 and Os PIP1;3 genes,and inserted a single T base in the Os PIP1;2 gene,respectively.While line 18 demonstrated an insertion of a single A base in the Os PIP1;1gene and a single T base in both the Os PIP1;2 and Os PIP1;3 genes,respectively.Multiplex editing of the Os PIP1 genes significantly inhibited photosynthetic rate and accumulation of compatible metabolites,but increased MDA contents and osmotic potentials in the mutants,thus delaying rice growth under salt stress.Functional loss of the Os PIP1 genes obviously suppressed the expressions of the Os PIP1,Os SOS1,Os CIPK24 and Os CBL4 genes,and increased the influxes of Na+and effluxes of K^(+)/H^(+)in the roots,thus accumulating more Na+in rice mutants under salt stress.This study suggests that the Os PIP1 genes are essential modulators collectively contributing to the enhancement of rice salt stress tolerance,and multiplex editing of the Os PIP1 genes provides insight into the osmotic regulation of the PIP genes.

关 键 词：AQUAPORIN Multiplex gene editing CRISPR/Cas9 OsPIP1 genes Rice(Oryza sativa L.) Salt tolerance 

分 类 号：S511[农业科学—作物学]