Flavonol synthase gene MsFLS13 regulates saline-alkali stress tolerance in alfalfa  

作　　者：Lishuang Zhang Yugang Sun Jinqiang Ji Weidi Zhao Weileng Guo Jiaqi Li Yan Bai Dan Wang Zhe Yan Changhong Guo 

机构地区：[1]Key Laboratory of Molecular and Cytogenetics,College of Life Science and Technology,Harbin Normal University,Harbin 150025,Heilongjiang,China [2]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《The Crop Journal》2023年第4期1218-1229,共12页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (U21A20182, 31972507);the Science and Technology Major Project of Heilongjiang Province (2021ZXJ03B05);the Graduate Innovation Fund of Harbin Normal University (HSDBSCX2021-106)。

摘　　要：Alfalfa(Medicago sativa L.) is one of the most extensively grown leguminous forage worldwide.Environmental saline-alkali stress significantly influences the growth,development,and yield of alfalfa,posing a threat to its agricultural production.However,little is known about the potential mechanisms by which alfalfa responds to saline-alkali stress.Here,we investigated these mechanisms by cloning a saline-alkali-induced flavonol synthase gene(Ms FLS13) from alfalfa,which was previously reported to be significantly upregulated under saline-alkali stress,and examining its function in the saline-alkali response.Overexpression of Ms FLS13 in alfalfa promoted plant tolerance to saline-alkali stress by enhancing flavonol accumulation,antioxidant capacity,osmotic balance,and photosynthetic efficiency.Conversely,Ms FLS13 inhibition using RNA interference reduced flavonol synthase activity and inhibited hairy root growth under saline-alkali stress.Yeast one-hybrid and dual-luciferase reporter assays indicated that the R2R3-MYB Ms MYB12 transcription factor activates Ms FLS13 expression by binding to the MBS motif in the Ms FLS13 promoter.Further analysis revealed that abscisic acid mediates the salinealkali stress response partially by inducing Ms MYB12 and Ms FLS13 expression,which consequently increases flavonol levels and maintains antioxidant homeostasis in alfalfa.Collectively,our findings highlight the crucial role of Ms FLS13 in alfalfa in response to saline-alkali stress and provide a novel genetic resource for creating saline-alkali-resistant alfalfa through genetic engineering.

关 键 词：ALFALFA SALINE-ALKALI Msfls13 Msmyb12 ANTIOXIDANT 

分 类 号：S541[农业科学—作物学]