蛋白质纳米笼介导的酶自固定化研究进展  

Advances in enzyme self-immobilization mediated by protein nanocages

作　　者：陈雅鑫 袁航 刘冠章 毛磊 杨纯 张瑞芳 张光亚[1] CHEN Yaxin;YUAN Hang;LIU Guanzhang;MAO Lei;YANG Chun;ZHANG Ruifang;ZHANG Guangya(College of Chemical Engineering,Huaqiao University,Xiamen 361021,Fujian,China)

机构地区：[1]华侨大学化工学院,福建厦门361021

出　　处：《化工学报》2023年第7期2773-2782,共10页CIESC Journal

基　　金：福建省自然科学基金项目(2020J01079)。

摘　　要：近年来,以金属有机框架以及自组装蛋白为代表的新型酶载体材料不断涌现,其中以蛋白质纳米笼为代表的自组装蛋白在酶自固定化领域的独特优势正不断被研究者发掘。蛋白质纳米笼独有的笼状空腔、可基因/化学修饰结构、可控自组装特性等特点为成功获得产量、稳定性和催化活性均提高的自固定化酶奠定了基础。本文首先简要介绍了金属有机框架材料、自组装蛋白,同时结合自身研究经历,重点对蛋白质纳米笼高级结构、人工设计尤其是铁蛋白的结构设计,以及纳米笼的自组装机制进行阐述。最后,综述了蛋白质纳米笼在酶自固定化方面的研究进展,并指出今后可能的研究方向,为建立自固定化新策略提供参考,进而推动自固定化酶从实验室研究迈向工业化应用。In recent years,new enzyme carrier materials represented by metal-organic frameworks and selfassembled proteins have emerged.Among them,the unique advantages of self-assembled proteins represented by protein nanocages in the field of enzyme self-immobilization are being continuously explored by researchers.Due to the unique cage-like cavity,facile gene/chemical modification and controllable self-assemble of protein nanocages,the self-immobilized enzyme with improved yield,stability and catalytic activity is readily achieved.This review begins with an overview of novel enzyme self-immobilization materials,such as metal-organic framework materials and self-assembled proteins.Then,the characteristics of nanocage proteins are presented in terms of 3D structure,particularly the structural design of ferritin and self-assembly mechanism.Finally,the research progress of protein nanocages in enzyme self-immobilization is reviewed,and the possible research directions in the future are pointed out,providing reference for establishing new self-immobilization strategies,and then promoting self-immobilized enzymes from laboratory research to industrial application.

关 键 词：蛋白质纳米笼 固定化 自组装 铁蛋白 酶 生物催化 

分 类 号：Q814[生物学—生物工程] TB383.1[一般工业技术—材料科学与工程]