‘黎平2号’油茶良种组培快繁技术  

Superior breeding of Camellia oleifera'Liping No.2'using tissue culture and rapid propagation

作　　者：陈胜群 王港 刘小红 沈莲文 田怀 CHEN Shengqun;WANG Gang;LIU Xiaohong;SHEN Lianwen;TIAN Huai(Guizhou Academy of Forestry,Guiyang 550005,Guizhou,P.R.China;Guizhou Institute of Walnut,Guiyang 550005,Guizhou,P.R.China;Guizhou Forestry School,Guiyang 550035,Guizhou,P.R.China)

机构地区：[1]贵州省林业科学研究院,贵州贵阳550005 [2]贵州省核桃研究所,贵州贵阳550005 [3]贵州省林业学校,贵州贵阳550035

出　　处：《贵州林业科技》2023年第3期14-19,共6页Guizhou Forestry Science and Technology

基　　金：贵州省科技厅优秀青年科技人才项目(黔科合平台人才[20195643]);贵州省林业局项目(‘黎平2号’油茶良种组培快繁技术研究)(黔林科合2022[34号]);贵州省林业局项目(威宁短柱油茶组培技术研究)(黔林科合2020[15号])。

摘　　要：为筛选一套便捷高效的‘黎平2号’油茶良种组培快繁技术体系以应用于生产,本研究以贵州小果油茶良种‘黎平2号’带芽茎段为试验材料,以MS为基本培养基,加入不同组合的植物激素进行‘黎平2号’油茶组培快繁技术研究。结果表明:MS+2.5 mg·L^(-1)6-BA+0.8 mg·L^(-1)NAA+0.08 mg·L^(-1)TDZ为‘黎平2号’不定芽诱导最佳培养基,MS+0.03 mg·L^(-1)NAA+0.02 mg·L^(-1)TDZ为最佳增殖培养基,1/2MS+0.5 mg·L^(-1)IBA+0.5 mg·L^(-1)NAA为‘黎平2号’油茶的最佳生根培养基,本研究结果将为贵州省油茶良种产业化发展提供种苗保障。An efficient and convenient system is highly required for the tissue culture and rapid propagation in recent years.This study aims to screen such a new system for the improved variety of'Liping No.2'Camellia oleifera in forest production.The experimental materials were taken as the sprouted stem segments of small fruit C.oleifera variety'Liping No.2'in Guizhou Province of China.Murashige and Skoog(MS)was then selected as the basic culture medium.Finally,an optimal combination of plant hormones was achieved for the better performance of the tissue culture and rapid propagation.The results showed that the MS+2.5 mg·L^(-1)6-BA+0.8 mg·L^(-1)NAA+0.08 mg·L^(-1)TDZ was the optimal medium for the inducing adventitious buds in'Liping No.2',while the MS+0.03 mg·L^(-1)NAA+0.02 mg·L^(-1)TDZ was the optimal proliferation medium,and 1/2MS+0.5 mg·L^(-1)IBA+0.5 mg·L^(-1)NAA was the optimal rooting medium for'Liping No.2'small fruit C.oleifera.The finding can provide a strong reference for the seed and seedling protection in the large-scale industrialization of C.oleifera in Guizhou Province of China.

关 键 词：黎平2号 油茶 组培快繁 

分 类 号：S722[农业科学—林木遗传育种]