硫自养强化PHBV异养反硝化脱氮的协同特性  

Synergistic Properties of Sulfur Autotrophic Enhanced PHBV Heterotrophic Denitrification for Nitrogen Removal

作　　者：葛丹丹 刘锋[1,2] 许国栋 张雪智 冯震 陈柯秀 GE Dan‐dan;LIU Feng;XU Guo‐dong;ZHANG Xue‐zhi;FENG Zhen;CHEN Ke‐xiu(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;Municipal Wastewater Utilization Technology National Local Joint Engineering Laboratory,Suzhou 215009,China;Suzhou Zhongsheng Environmental Remediation Limited Company,Suzhou 215009,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215009 [2]城市生活污水资源化利用技术国家地方联合工程实验室,江苏苏州215009 [3]苏州中晟环境修复有限公司,江苏苏州215009

出　　处：《中国给水排水》2023年第13期16-24,共9页China Water & Wastewater

基　　金：江苏省研究生科研创新计划项目(KYCX20_2765);苏州市水利水务科技项目(2019006)。

摘　　要：在基于聚羟基丁酸酯(PHBV)的反硝化填充床反应器中,引入硫代硫酸盐作为自养反硝化电子供体强化反硝化脱氮。构建了两套PHBV反硝化反应器(R1和R2),分别针对不同水力停留时间(HRT),探讨了投加硫代硫酸盐强化反硝化脱氮性能的协同效应。结果表明,当进水NO_(3)^(-)-N浓度为50 mg/L时,将R1和R2的HRT分别从1.0、1.5 h缩短至为0.5、0.75 h,出水NO_(3)^(-)-N浓度升高至26.18、18.78 mg/L,NO_(2)^(-)-N也发生了积累。向两个反应器中分别加入25、50和75 mg/L的硫代硫酸盐,NO_(3)^(-)-N去除率逐渐提高。当HRT为0.75 h、硫代硫酸盐加入量为50 mg/L时,反硝化协同效应显著,NO_(3)^(-)-N去除率达到了100%,出水pH在6.8~7.4之间、SO_(4)^(2-)-S<250 mg/L。通过扫描电镜观察发现,在微生物的水解作用下PHBV表面出现了无数孔隙,为微生物的附着生长提供了空间。高通量测序分析结果显示,两个反应器内的功能菌属主要包括Thiobacillus、Comamonadaceae和Thermomonas等。Thiosulfate was introduced as an electron donor for autotrophic denitrification to enhance nitrogen removal in a denitrifying biofilter packed with polyhydroxybutyrate(PHBV).This paper constructed two sets of denitrifying biofilter packed with PHBV(R1 and R2),and investigated the synergistic effect of adding thiosulfate to enhance nitrogen removal performance according to different hydraulic retention time(HRT).When the nitrate nitrogen in influent was 50 mg/L,and the HRTs of R1 and R2 were shortened from 1.0 h and 1.5 h to 0.5 h and 0.75 h respectively,the nitrate nitrogen in effluent increased to 26.18 mg/L and 18.78 mg/L respectively accompanied with the accumulation of nitrite nitrogen.The removal rate of nitrate nitrogen gradually increased after adding 25 mg/L,50 mg/L and 75 mg/L thiosulfate to the two reactors,respectively.When the HRT was 0.75 h and the thiosulfate dosage was 50 mg/L,the denitrification synergistic effect was significant,the nitrate nitrogen removal rate reached 100%,the effluent pH was between 6.8 and 7.4,and the SO_(4)^(2-)-S concentration was less than 250 mg/L.Scanning electron microscopy revealed that numerous voids appeared on the surface of PHBV under the action of microbial hydrolysis,which provided habitats for the adhesion and growth of microorganisms.High‐throughput sequencing results showed that the functional bacterial genera in the two reactors mainly included Thiobacillus,Comamonadaceae and Thermomonas.

关 键 词：固态有机碳源 聚羟基丁酸酯 硫代硫酸盐 异养反硝化 硫自养反硝化 高通量测序 

分 类 号：TU992[建筑科学—市政工程]