Enlarged fins of Tibetan catfish provide new evidence of adaptation to high plateau  

作　　者：Liandong Yang Ning Sun Honghui Zeng Ying Wang Wenjun Chen Zufa Ding Yang Liu Jing Wang Minghui Meng Yanjun Shen Jingliang Kang Xiuhui Ma Wenqi Lv Juan Chen Axel Meyer Baocheng Guo Shunping He 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072,China [2]Key Laboratory of Zoological Systematics and Evolution,Institute of Zoology,Chinese Academy of Sciences,Beijing,100101,China [3]Academy of Plateau Science and Sustainability,Qinghai Normal University,Xining,810016,China [4]Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin,School of Life Sciences,Jianghan University,Wuhan,430056,China [5]State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,650223,China [6]University of Chinese Academy of Sciences,Beijing,100049,China [7]Department of Biology,University of Konstanz,78457,Konstanz,Germany [8]Center for Excellence in Animal Evolution and Genetics,Chinese Academy of Sciences,Kunming,650223,China

出　　处：《Science China(Life Sciences)》2023年第7期1554-1568,共15页中国科学（生命科学英文版）

基　　金：supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDB31000000);the National Natural Science Foundation of China(32170480,31972866,31702016,31601858,32022009);Chinese Academy of Sciences(Youth Innovation Promotion Association),Chinese Academy of Sciences(http://www.yicas.cn);the Pioneer Hundred Talents Program,and ZDBS-LYSM005);the Second Tibetan Plateau Scientific Expedition and Research Program(STEP,2019QZKK0501);State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences(GREKF21-04);the Young Top-notch Talent Cultivation Program of Hubei Province;supported by the Wuhan Branch,Supercomputing Center,Chinese Academy of Sciences,China。

摘　　要：The uplift of the Tibetan Plateau significantly altered the geomorphology and climate of the Euroasia by creating large mountains and rivers.Fishes are more likely to be affected relative to other organisms,as they are largely restricted to river systems.Faced with the rapidly flowing water in the Tibetan Plateau,a group of catfish has evolved greatly enlarged pectoral fins with more numbers of fin-rays to form an adhesive apparatus.However,the genetic basis of these adaptations in Tibetan catfishes remains elusive.In this study,we performed comparative genomic analyses based on the chromosome-level genome of Glyptosternum maculatum in family Sisoridae and detected some proteins with conspicuously high evolutionary rates in particular in genes involved in skeleton development,energy metabolism,and hypoxia response.We found that the hoxd12a gene evolved faster and a loss-of-function assay of hoxd12a supports a potential role for this gene in shaping the enlarged fins of these Tibetan catfishes.Other genes with amino acid replacements and signatures of positive selection included proteins involved in low temperature(TRMU)and hypoxia(VHL)responses.Functional assays reveal that the G.maculatum TRMU allele generates more mitochondrial ATP than the ancestral allele found in low-altitude fishes.Functional assays of VHL alleles suggest that the G.maculatum allele has lower transactivation activity than the low-altitude forms.These findings provide a window into the genomic underpinnings of physiological adaptations that permit G.maculatum to survive in the harsh environment of the Tibetan Himalayas that mirror those that are convergently found in other vertebrates such as humans.

关 键 词：Glyptosternum maculatum enlarged fins ADAPTATION Tibetan Plateau 

分 类 号：Q958.8[生物学—动物学]