The patterns and participants of parental histone recycling during DNA replication in Saccharomyces cerevisiae  

作　　者：Zhongqing Shan Yan Zhang Jiachen Bu Huizhi Li Zhuqiang Zhang Jun Xiong Bing Zhu 

机构地区：[1]National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing,100101,China [2]College of Life Sciences,University of Chinese Academy of Sciences,Beijing,100049,China

出　　处：《Science China(Life Sciences)》2023年第7期1600-1614,共15页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China(2019YFA0508900);the National Natural Science Foundation of China(32170608,32288102);the K.C.Wong Educational Foundation(GJTD-2020-06);supported by the Youth Innovation Promotion Association(2017133,2020097)of the Chinese Academy of Sciences。

摘　　要：Epigenetic information carried by histone modifications not only reflects the state of gene expression,but also participates in the maintenance of chromatin states and the regulation of gene expression.Recycling of parental histones to daughter chromatin after DNA replication is vital to mitotic inheritance of epigenetic information and the maintenance of cell identity,because the locus-specific modifications of the parental histones need to be maintained.To assess the precision of parental histone recycling,we developed a synthetic local label-chasing system in budding yeast Saccharomyces cerevisiae.Using this system,we observed that parental histone H3 can be recycled to their original position,thereby recovering their position information after DNA replication at all tested loci,including heterochromatin boundary,non-transcribed region,and actively transcribed regions.Moreover,the recycling rate appears to be affected by local chromatin environment.We surveyed a number of potential regulatory factors and observed that histone H3-H4 chaperon Asf1 contributed to parental histone recycling,while the eukaryotic replisome-associated components Mcm2 and Dpb3 displayed compounding effects in this process.In addition,the FACT complex also plays a role in the recycling of parental histones and helps to stabilize the nucleosomes.

关 键 词：epigenetic inheritance parental histone recycling Asf1 MCM2 Dpb3 FACT 

分 类 号：Q75[生物学—分子生物学]