TaMADS29 interacts with TaNF-YB1 to synergistically regulate early grain development in bread wheat  

作　　者：Guoyu Liu Runqi Zhang Sen Li Rehmat Ullah Fengping Yang Zihao Wang Weilong Guo Mingshan You Baoyun Li Chaojie Xie Liangsheng Wang Jie Liu Zhongfu Ni Qixin Sun Rongqi Liang 

机构地区：[1]Frontiers Science Center for Molecular Design Breeding(MOE),State Key Laboratory for Agrobiotechnology,State Key Laboratory of Plant Physiology and Biochemistry,Key Laboratory of Crop Heterosis and Utilization(MOE),and Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing,100193,China

出　　处：《Science China(Life Sciences)》2023年第7期1647-1664,共18页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFF1002902,2016YFD0100803)。

摘　　要：Grain development is a crucial determinant of yield and quality in bread wheat(Triticum aestivum L.).However,the regulatory mechanisms underlying wheat grain development remain elusive.Here we report how Ta MADS29 interacts with Ta NF-YB1 to synergistically regulate early grain development in bread wheat.The tamads29 mutants generated by CRISPR/Cas9 exhibited severe grain filling deficiency,coupled with excessive accumulation of reactive oxygen species(ROS)and abnormal programmed cell death that occurred in early developing grains,while overexpression of Ta MADS29 increased grain width and1,000-kernel weight.Further analysis revealed that Ta MADS29 interacted directly with Ta NF-YB1;null mutation in Ta NF-YB1caused grain developmental deficiency similar to tamads29 mutants.The regulatory complex composed of Ta MADS29 and Ta NF-YB1 exercises its possible function that inhibits the excessive accumulation of ROS by regulating the genes involved in chloroplast development and photosynthesis in early developing wheat grains and prevents nucellar projection degradation and endosperm cell death,facilitating transportation of nutrients into the endosperm and wholly filling of developing grains.Collectively,our work not only discloses the molecular mechanism of MADS-box and NF-Y TFs in facilitating bread wheat grain development,but also indicates that caryopsis chloroplast might be a central regulator of grain development rather than merely a photosynthesis organelle.More importantly,our work offers an innovative way to breed high-yield wheat cultivars by controlling the ROS level in developing grains.

关 键 词：CHLOROPLAST grain filling reactive oxygen species TaMADS29 TaNF-YB1 wheat(Triticum aestivum L.) 

分 类 号：S512.1[农业科学—作物学]