合成大麻素MDMB-4en-PICA的体内和体外代谢研究  

Metabolism of synthetic cannabinoid MDMB-4en-PICA in vivo and in vitro.

作　　者：占志胜 张文芳 刘华 古锟山 徐多麒 张凯[5] Zhan Zhisheng;Zhang Wenfang;Liu Hua;Gu Kunshan;Xu Duoqi;Zhang Kai(College of forensic medicine,Shanxi Medical University,Key Laboratory of forensic Medicine in Shanxi Province,Shanxi 030600,China;Forensic Science Service of Beijing Public Security Bureau.Key Laboratory of Forensic Toxicology,Ministry of Public Security,Beijing 100192,China;School of Investigation,People's Public Security University of China,Beijing 100038,China;Shanghai Key Lab of Forensic Medicine,Key Lab of Forensic Science,Ministry of Justice,China(Academy of Forensic Science),Shanghai 200063,China;Narcotics Control Bureau of Tianjin Public Security Bureau,Tianjin 300240,China)

机构地区：[1]山西医科大学法医学院,法医学山西省重点实验室,山西晋中030600 [2]北京市公安司法鉴定中心,法庭毒物分析公安部重点实验室,北京100192 [3]中国人民公安大学侦查学院,北京100038 [4]上海市法医学重点实验室,司法部司法鉴定重点实验室(司法鉴定科学研究院),上海200063 [5]天津市公安局禁毒总队,天津300240

出　　处：《中国法医学杂志》2023年第3期262-267,共6页Chinese Journal of Forensic Medicine

基　　金：北京市公安局技术研究科研项目(2022CX1001)。

摘　　要：目的随着新型合成大麻素的演变,其在体内代谢速度增快,对原药的检测难度增加,为确定是否吸食合成大麻素,其生物标志物可以作为强有力的证据,常见的代谢模型有体内代谢模型和体外代谢模型。方法本研究采用液相色谱-高分辨质谱技术检测斑马鱼体内代谢模型和人肝微粒体体外代谢模型中MDMB-4en-PICA的代谢情况,并使用Trance Finder 4.1通用版进行数据采集和Compound Discoverer 2.1版本进行代谢物鉴定。结果斑马鱼模型代谢产生26种代谢物,人肝微粒体模型代谢产生17种代谢物,总共32种代谢物,其中包括23种Ⅰ相代谢物和9种Ⅱ相代谢物,涉及10种代谢途径。结论结果显示,两种模型中二氢二醇代谢物(M16)的峰面积均最高,其次是羟基化代谢物(M22),而且具有一定的特异性,可以推荐作为MDMB-4en-PICA潜在的生物标志物。Objective With the evolution of synthetic cannabinoids,its metabolic rate in vivo become faster,and it is more difficult to detect the original drug.In order to determine whether a certain type of synthetic cannabinoids is ingested,its biomarkers can be used as compelling evidence.Common metabolic models include in vivo metabolic model and in vitro metabolic model.Methods In this study,liquid chromatography-high resolution mass spectrometry was used to detect the metabolism of MDMB-4en-PICA in zebrafish in vivo metabolism model and human liver microsome in vitro metabolism model.Trance Finder 4.1 general version was used for data collection and Compound Discoverer 2.1 version was used for metabolite identification.Results Metabolism of zebrafish model yielded 26 metabolites,and metabolism of human liver microsome model yielded 17 metabolites,A total of 32 metabolites were detected,including 23 phase I metabolites and 9 phase II metabolites,involving 10 metabolic pathways.Conclusion The results showed that dihydrodiol metabolite(M16)had the highest peak area in both models,followed by hydroxylated metabolite(M22),and it has certain specificity,which can be recommended as a potential biomarker of MDMB-4en-PICA.

关 键 词：LC-HRMS 体内外代谢模型 代谢物 生物标志物 

分 类 号：R918.93[医药卫生—药学]