盐胁迫下乌桕无性系叶片的比较蛋白组学研究  

Comparative Proteomics on Leaves of Triadica sebifera Clones under Salt Stress

作　　者：隋德宗[1] 王保松[1] SUI Dezong;WANG Baosong(Jiangsu Academy of Forestry,Nanjing 211153)

机构地区：[1]江苏省林业科学研究院,南京211153

出　　处：《植物研究》2023年第5期679-689,共11页Bulletin of Botanical Research

基　　金：江苏现代农业重点项目(BE2018401);江苏省林业科学研究院自主科研项目(ZZKY202103);江苏省林业科技创新与推广项目(LYKJ202108)。

摘　　要：为探讨乌桕(Triadica sebifera)蛋白水平响应盐胁迫的分子机制,研究选取盐敏感型无性系(SS18)和盐耐受型无性系(ST21),采用0.4%NaCl浇灌模拟盐胁迫,利用同位素标记相对和绝对定量(iTRAQ)技术对胁迫不同时期(0、24、72 h)叶片蛋白进行定量。在SS18和ST21中分别鉴定出显著差异蛋白279、106种。盐胁迫条件下2个无性系中共同存在4种(过氧化氢酶、延伸因子-1α、含H+-ATPase_c结构域的蛋白和硫氧还蛋白)显著上调表达蛋白,推测其可能是乌桕耐盐响应的重要潜在靶标蛋白。通路富集分析发现乌桕叶片响应盐胁迫的差异蛋白主要与光合作用、糖类代谢、氨基酸代谢、脂肪酸代谢途径有关。蛋白-蛋白互作网络发现SS18中分别存在5个(24 h)和3个(72 h)参与糖分解及能量代谢的核心蛋白;而ST21中存在5个(24 h)和4个(72 h)参与碳代谢、光合固碳、光合作用代谢及与叶绿素a-b结合相关的核心蛋白。ST21叶片通过提高糖类、氨基酸及脂肪酸代谢途径,并积累大量可溶性糖、氨基酸和有机酸等小分子可溶性物质,这可能是其参与盐胁迫响应的重要方面。In order to investigate the molecular mechanisms of protein responses in Triadica sebifera to salt stress,two T.sebifera lines,including salt-sensitive P18(SS18)and salt-tolerant P21(ST21)were selected and treated with 0.4%NaCl solution.Isotope tags for relative and absolute quantitation(iTRAQ)were used to quantify leaf proteins abundance changes at different stress periods(0,24,72 h).A total of 279 and 106 differentially expressed proteins(DEPs)were detected in SS18 and ST21,respectively.Four proteins,including catalase,extension factor 1-α,H+-ATPase c domain protein and thioredoxin,were significantly upregulated in two clones under salt stress,suggesting that they may be important potential target proteins in the salt-tolerant response of T.sebifera’s.Pathway enrichment analysis showed that the DEPs of T.sebifera leaves under salt stress were primarily associated with photosynthesis,carbohydrate metabolism,amino acid metabolism,and fatty acid metabolism.The protein-protein interaction network showed that 5 core proteins(24 h)and 3 core proteins(72 h)involved in glucose decomposition and energy metabolism in SS18,while ST21 had 5(24 h)and 4(72 h)core proteins involved in carbon metabolism,photosynthesis,carbon fixation,photosynthesis and related to chlorophyll a-b binding.ST21 leaves improved metabolic pathways of sugars,

关 键 词：乌桕 叶片 盐胁迫 ITRAQ 

分 类 号：S793.9[农业科学—林木遗传育种]