霸王鞭内生真菌Fusarium sp.DCJ-A发酵物的次生代谢产物研究  

Study on the secondary metabolites of endophytic fungus Fusarium sp.DCJ-A from Euphorbia royleana Boiss

作　　者：杨家梅 黄磊 陈宁 廖永剑 张力之 周敏[1] YANG Jia-mei;HUANG Lei;CHEN Ning;LIAO Yong-jian;ZHANG Li-zhi;ZHOU Min(Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission&Ministry of Education,Yunnan Minzu University,Kunming 650500,China)

机构地区：[1]云南民族大学民族药资源化学国家民族事务委员会-教育部重点实验室,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2023年第4期397-402,共6页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(31860099);云南省自然科学基金(202001AW07002).

摘　　要：采用多种色谱方法对分离自大戟属植物霸王鞭(Euphorbia royleana Boiss)的内生真菌Fusarium sp.DCJ-A的发酵物进行提取和分离,所得化合物的结构通过核磁共振波谱法进行了结构解析,共鉴定了8个化合物,其中包括2个含氧杂环化合物、3个含氮杂环化合物以及3个苯环衍生物,分别为:1 H-imidazole-4-carboxylic acid(1)、4-methlpyrazole-3(5)-carboxylic acid(2)、p-hydroxy phenylacetic acid(3)、methyl-2-(4-hydroxyphenyl)acetate(4)、methyl-4-butyranmiddobenzoate(5)、6-(1-hydroxypentyl)-4-methoxy-2 H-pyran-2-one(6)、(3 S,6R)-6-(l-methylethyl)-3-phenylmethyl-l,4-perhydrooxazine-2,5-dione(7)和xylophone(8),其中化合物2、5、7和8共4个化合物均首次从镰刀菌属Fusarium中分离得到.Eight compounds were isolated and extracted from the fermentation of endophytic fungus Fusarium sp.DCJ-A from Euphorbia royleana Boiss by chromatographic methods,including two oxygen-containing heterocyclic compounds,three nitrogen-containing heterocyclic compounds and three benzene ring derivatives.The structures of these compounds were elucidated by the basis of spectroscopy methods,and identified as:1 H-imidazole-4-carboxylic acid(1),4-methlpyrazole-3(5)-carboxylic acid(2),p-hydroxyphenylacetic acid(3),methyl-2-(4-hydroxyphenyl)acetate(4),methyl-4-butyranmiddobenzoate(5),6-(1-hydroxypentyl)-4-methoxy-2 H-pyran-2-one(6),(3S,6R)-6-(l-methylethyl)-3-phenylmethyl-l,4-perhydrooxazine-2,5-dione(7),and xylophone(8).Among them,compounds 2、5、7 and 8 are isolated from Fusarium for the first time.

关 键 词：霸王鞭 内生真菌 Fusarium sp. 杂环化合物 

分 类 号：R284.1[医药卫生—中药学]