铜砭刮痧联合耳穴压豆对MCAO大鼠脑缺血损伤改善效果及炎性因子的影响  

Effects of gua sha combined with ear acupuncture pressure beans on the improvement effect and inflammatory factors of cerebral ischemia injury in MCAO rats

作　　者：王小亮[1] 邱航健 李振东 王晓倩 张诚诚 张月娟[1] WANG Xiao-liang;QIU Hang-jian;LI Zhen-dong;WANG Xiao-qian;ZHANG Cheng-cheng;ZHANG yue-juan(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Human Province 410007,China;School of Medicine,Human University of Chinese Medicine,Changsha,Human Province 410013,China)

机构地区：[1]湖南中医药大学第一附属医院护理部,湖南长沙410007 [2]湖南中医药大学护理学院,湖南长沙410006

出　　处：《解剖学研究》2023年第3期223-229,共7页Anatomy Research

基　　金：湖南省卫健委科研计划课题(202114011882);湖南省重点科研计划项目(202004);湖南省科技厅临床引导项目(2020SK51401);湖南省中医药管理局中医药科研课题(B2023119)。

摘　　要：目的 探讨铜砭刮痧联合耳穴压豆对大脑中动脉闭塞(MCAO)大鼠脑缺血损伤改善效果及炎症因子的影响。方法 将50只雄性SD大鼠随机分为假手术组、模型组、刮痧组、耳穴组、联合组,每组10只,采用大脑中动脉线栓法建立MCAO模型。与造模d1开始,对大鼠进行刮痧及耳穴等干预,于干预14 d后,采用mNSS评分表评估大鼠神经功能缺损程度,ELISA检测血清中炎性因子TNF-α、IL-1β、IL-6、IL-10的含量,TTC染色法测定脑梗死面积,Western blot法检测脑组织IL-6、IL-10蛋白含量,RT-qPCR检测IL-6、IL-10 m RNA表达水平。结果 刮痧组、耳穴组、联合组在mNSS评分、脑梗死面积方面均优于模型组(P<0.05)。刮痧组、联合组血清TNF-α、IL-1β、IL-6含量低于模型组(P<0.05),IL-10高于模型组(P<0.05),蛋白含量及mRNA表达IL-6低于模型组(P<0.05),IL-10高于模型组(P<0.05)。耳穴组血清TNF-α、IL-1β、IL-6含量低于模型组(P<0.05),但IL-10含量差异无统计学意义(P>0.05);蛋白含量及mRNA表达IL-6低于模型组(P<0.05),但IL-10差异无统计学意义(P>0.05)。结论 铜砭刮痧联合耳穴压豆可改善MCAO大鼠神经功能,及作用可能与免疫调节机制有关。Objective To investigate the effect of gua sha combined with auricular pressure beans on the improvement effect and inflammatory factors of cerebral ischemia injury in MCAO rats.Methods 50 male SD rats were randomly divided into sham surgery group,model group,gua sha group,ear acupuncture group,and joint group,each group of 10 rats,and the MCAO model was established by the middle cerebral artery line embolism method.Starting with mold d1,rats were treated with gua sha and ear acupuncture and other interventions,and after 14 days of intervention,the degree of neurologic defects in rats was assessed by mNSS scoring table,ELISA detect⁃ed the content of inflammatory factors TNF⁃α,IL⁃1β,IL⁃6 and IL⁃10 in serum,TTC staining determined the area of cerebral infarction,Western blot method detected brain tissue IL⁃6,IL⁃10 protein content,RT⁃qPCR detected IL⁃6,IL⁃10 mRNA expression levels.Results The Gua Sha group,ear acupuncture group and combined group were bet⁃ter than the model group in terms of mNSS score and cerebral infarction area(P<0.05).The serum TNF⁃α,IL⁃1βand IL⁃6 levels in the Gua Sha group and the combined group were lower than those in the model group(P<0.05),IL⁃10 was higher than that in the model group(P<0.05),the protein content and mRNA expression IL⁃6 were lower than in the model group(P<0.05),and the IL⁃10 was higher than that in the model group(P<0.05).The serum TNF⁃α,IL⁃1βand IL⁃6 content in the auricular group were lower than those in the model group(P<0.05),but there was no statistical difference in IL⁃10 content(P>0.05),and the protein content and mRNA expression IL⁃6 were lower than in the model group(P<0.05),but there was no statistical difference in IL⁃10(P>0.05).Conclu⁃sion Gua Sha combined with ear acupuncture pressure beans improves nerve function in MCAO rats,and its effect may be related to immunomodulatory mechanisms.

关 键 词：铜砭刮痧 耳穴压豆 脑卒中 大脑中动脉闭塞 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]