辣椒疫霉病菌拮抗菌的筛选鉴定及生防潜力  

Screening and identification of antagonistic strain against Phytophthora capsici and the biocontrol potential

作　　者：郑迪文 周游 谢芳玲 谢宗宝 刘峰 朱宏建[1] ZHENG Diwen;ZHOU You;XIE Fangling;XIE Zongbao;LIU Feng;ZHU Hongjian(College of Plant Protection,Hunan Agricultural University,Changsha,Hunan 410128,China;Guangdong Laboratory for Lingnan Modern Agricultural,Guangzhou,Guangdong 510006,China;College of Horticulture,Hunan Agricultural University,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]岭南现代农业科学与技术广东省实验室,广东广州510006 [3]湖南农业大学园艺学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2023年第4期442-447,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：岭南现代农业科学与技术广东省实验室项目(NT2021004);湖南省科学技术厅麓山种业项目(2021NK1006);湖南省教育厅高校科技创新平台建设项目(20K071)。

摘　　要：从湖南省长沙、衡阳、岳阳等地辣椒种植区土样中分离筛选出6株拮抗辣椒疫霉病菌的放线菌,采用含毒介质法检测,发现L57-60菌株发酵液的5倍稀释液对辣椒疫霉菌的抑制效果达到98.61%;通过形态学特征观察、生理生化特性鉴定以及16S rDNA、recA基因、atpD基因和rpo B基因联合建树分析,确定L57-60菌株为橄榄色链霉菌(Streptomycesolivaceus)。抗菌谱测定结果表明,L57-60菌株发酵液对辣椒枯萎病菌(Fusarium oxysporum)、烟草炭疽病菌(Colletotrichummicotianae)、水稻纹枯病菌(Thanatephoruscucumeris)等12种病原菌均有拮抗作用。运用正交试验对L57-60菌株发酵培养基的碳源和氮源配比进行优化,结果最适培养基为高氏1号培养基,大米粉质量分数4%,黄豆粉质量分数0.5%。盆栽试验结果表明,L57-60菌株发酵液对辣椒疫霉的防效达到73.1%。Six strains of antagonistic actinomycetes against Phytophthora capsici were isolated and screened from soil samples of pepper planting areas in Changsha,Hengyang and Yueyang,Hunan Province.The results showed that the 5times sterile dilution of the fermentation broth of strain L57-60 had 98.61% inhibition effect on Phytophthora capsici.Strain L57-60 was identified as Streptomyces olivaceus by morphological characteristics,physiological and biochemical identification,combined with joint tree established based on 16S r DNA,recA,atpD and rpo B genes.Antimicrobial spectrometry showed that fermentation broth of strain L57-60 showed antagonistic effect on 12 pathogens including Fusarium oxysporum in pepper,Colletotrichum micotianae in tobacco and Thanatephorus cucumeris(Frank) Donk.The fermentation medium of strain L57-60 was optimized by orthogonal experiment.The results showed that the optimal fermentation medium formula for strain L57-60 was composed of corn flour 4%,soybean flour 0.5%.Pot experiment showed the control effect of strain L57-60 against Phytophthora capsici was 73.1%.

关 键 词：辣椒疫病 橄榄色链霉菌 发酵培养基优化 盆栽防效 

分 类 号：S436.418.1[农业科学—农业昆虫与害虫防治]