微量磺胺甲恶唑对饮用水管网生物膜群落及抗性基因的影响与控制  

Influence of micro-sulfamethoxazole on the biofilm bacterial communities and antibiotic resistance genes in drinking water distribution systems and its control

作　　者：胡炽盛 马徐 倪炯 王亮 王海波[1,2] 石宝友 HU Chisheng;MA Xu;NI Jiong;WANG Liang;WANG Haibo;SHI Baoyou(Key Laboratory of Drinking Water Science and Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;University of Chinese Academy of Sciences,Beijing 100049,China;Shaoxing Shangyu water supply company,Shaoxing 312300,China)

机构地区：[1]中国科学院生态环境研究中心饮用水科学与技术重点实验室,北京100085 [2]中国科学院大学,北京100049 [3]绍兴市上虞区供水有限公司,绍兴312300

出　　处：《环境工程学报》2023年第6期2077-2087,共11页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(52070189,51838005);国家重点研发计划项目(2019YFD1100105)。

摘　　要：针对饮用水管网系统可能存在的微生物风险问题,采用模拟不同处理条件下的输配水管道系统,通过宏基因组学分析探究微量磺胺甲恶唑以及次氯酸钠消毒对管道中生物膜与抗性基因组成的影响。结果表明,2μg·L^(-1)磺胺甲恶唑的添加对微生物群落以及抗性基因组成无明显影响,而浮霉菌门细菌表现出很强的抗次氯酸钠消毒能力。在未消毒条件下丰度前十的抗性基因与携带差异性抗性基因的细菌在消毒后丰度均明显有所下降,次氯酸钠消毒使ARGs总量下降了91.9%,因此,次氯酸钠消毒通过控制携带抗性基因物种从而有效控制群落抗性基因的传播。同时,通过组间显著性差异的功能基因与组间显著性差异的抗性基因相关性分析,功能基因的变化情况与抗性基因变化情况一致,因此,长期消毒改变了细菌群落组成及其功能,并最终影响抗性基因传播。这项研究有助于控制长期运行的饮用水管网输配系统中可能存在的包括抗药基因在内的微生物相关风险问题。For the microbial risk in the water of drinking water distribution systems(DWDSs),metagenomic analysis was used to investigate the effects of micro-sulfamethoxazole and NaClO disinfection on biofilm bacterial communities and antibiotic resistance genes(ARGs) in the simulated DWDSs under different treatments.The experimental results showed that the bacterial communities and ARGs did not change significantly with addition of 2 μg-L^(-1) sulfamethoxazole,while the bacteria of Planctomycetes at phylum level showed a strong resistance to NaClO.The abundance of top ten ARGs and the bacteria carrying differential ARGs detected before disinfection both decreased significantly after disinfection.The total abundance of ARGs decreased by 91.9% due to the influence of NaClO.Therefore,the spread of ARGs was controlled by the NaClO disinfection because of its inhibition on the bacterial species carrying ARGs growth.Meanwhile,the correlation analysis between function genes and ARGs with significant differences among groups showed that the changes in function genes were consistent with changes in ARGs.Therefore,long disinfection changed the bacterial communities and their function,which affected the propagation of different ARGs.This study was contributed to the control of possible microbial risk including the ARGs in the water of long-running DWDSs.

关 键 词：磺胺甲恶唑 饮用水管网输配系统 细菌群落组成 抗生素抗性基因 宏基因组学 

分 类 号：X703[环境科学与工程—环境工程]