小核糖核酸调控蚊虫代谢解毒酶基因表达的研究进展  

Research progress on sRNA regulation of mosquito metabolic detoxification enzyme gene expression

作　　者：武佳慧 宋晓 彭荟 公茂庆[1] WU Jiahui;SONG Xiao;PENG Hui;GONG Maoqing(Shandong Institute of Parasitic Diseases,Shandong First Medical University(Shandong Academy of Medical Sciences),Jining,Shandong 2720332,China;Department of Healthcare-associated Infection Management,Liaocheng Brain Hospital,Liaocheng,Shandong 252000,China)

机构地区：[1]山东省寄生虫病防治研究所,山东第一医科大学(山东省医学科学院),山东济宁2720332 [2]山东省聊城市脑科医院感染管理科,山东聊城252000

出　　处：《中国热带医学》2023年第7期766-772,共7页China Tropical Medicine

基　　金：国家自然科学基金(No.81871685)。

摘　　要：随着分子生物学的发展,研究发现非编码小核糖核酸(small ribonucleic acid, sRNA)在基因表达及蛋白质活性方面具有重要的调控作用,影响着包括蚊虫对杀虫剂抗药性在内的多种生物学途径。了解抗药性的分子调控机制对控制蚊虫至关重要。其中代谢抗性是最关键的机制,主要指蚊虫体内的代谢解毒酶(特别是细胞色素P450酶系)相关基因的高表达。在已验证抗药性基因的基础上进一步研究sRNA与其之间的联系,为进一步阐明蚊虫抗药性发生机制提供了新的思路和方向。蚊虫代谢抗性机制的研究对病媒蚊虫治理、抗药性监测及新型杀虫剂研发意义重大。本文就抗药性基因、sRNA的生物合成、参与调控蚊虫代谢解毒酶基因及实际应用的研究进展予以综述。With the development of molecular biology,non-coding sRNA has been found to play an important regulatory role in gene expression and protein activity,affecting various biological pathways including mosquito resistance against insecticides.Understanding the molecular regulatory mechanisms of drug resistance is essential for controlling mosquitoes,,of which metabolic resistance being the most critical mechanism,mainly referring to the high expression of metabolic detoxification enzyme-related genes(especially the cytochrome P450 enzyme system)in mosquitoes.On the basis of verification of insecticide resistance-related genes,further research on the correlation between sRNA and mosquito resistancerelated genes provides new ideas and directions for further exploring the mechanism of mosquito resistance.The study of mosquito metabolic resistance mechanism is of great significance for the control of vector mosquitoes,drug resistance monitoring and novel insecticide development.This article reviews the progress of research on the resistance genes,sRNAs biosynthesis,genes involved in regulating mosquito metabolic detoxification enzymes and their applications.

关 键 词：小核糖核酸 微小核糖核酸 小干扰核糖核酸 与piwi蛋白相作用的核糖核酸 蚊虫 抗药性 

分 类 号：R384.1[医药卫生—医学寄生虫学]