猕猴桃根抗结肠癌活性提取物的筛选及其成分分析  

Screening and component analysis of Actinidia chinensis Radix extract with anti colon cancer activity

作　　者：梁光平 胡恩明 唐文茜 梁光焰 杨俊 崔婷 LIANG Guangping;HU Enming;TANG Wenqian;LIANG Guangyan;YANG Jun;CUI Ting(Department of Pharmacy,Zunyi Medical and Pharmaceutical College,Zunyi 563006,Guizhou,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,Guizhou,China)

机构地区：[1]遵义医药高等专科学校药学系,贵州遵义563006 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014

出　　处：《生物资源》2023年第3期250-258,共9页Biotic Resources

基　　金：贵州省科学技术基金资助项目(黔科合基础-ZK[2022]一般675)。

摘　　要：采用系统溶剂提取法制备了猕猴桃根石油醚、二氯甲烷、乙酸乙酯、正丁醇、水等5类提取物,并通过噻唑蓝法(methyl thiazolyl tetrazolium,MTT)评价它们对5种结肠癌细胞(SW480、HCT⁃116、HCT⁃15/Taxol、HCT⁃8/V、HCT⁃116/5⁃FU)和人正常结肠上皮细胞(FHC)的抑制作用。结果显示,猕猴桃根石油醚提取物对SW480、HCT⁃8/V细胞的作用最突出,IC50分别为(16.13±1.22)μg/mL、(21.61±1.46)μg/mL,且具有较高的选择指数(SISW480=16.93,SIHCT⁃8/V=12.64)。同时,采用液质联用技术从石油醚提取物中分析鉴定出了31种成分,其中包括20种萜类,5种香豆素和6种有机酸。Five kinds of extracts from Actinidia chinensis Radix were prepared by systematic solvent extraction meth⁃od,including petroleum ether,dichloromethane,ethyl acetate,n⁃butanol and water.Subsquently,the inhibitory effects of the five extracts on SW480,HCT⁃116,HCT⁃15/Taxol,HCT⁃8/V,HCT⁃116/5⁃FU and FHC cells were evaluated by methyl thiazolyl tetrazolium(MTT).The results showed that the five extracts could inhibit the growth of 5 kinds of tumor cells(SW480,HCT⁃116,HCT⁃15/Taxol,HCT⁃8/V,HCT⁃116/5⁃FU).The petroleum ether extract had the most prominent effect on SW480 cells(IC50=16.13±1.22μg/mL)and HCT⁃8/V cells(IC50=21.61±1.46μg/mL)with an ideal selection index,respectively.Simultaneously,31 components,including 20 terpenes,5 coumarins and 6 or⁃ganic acids,were identified from the petroleum ether extract by HPLC⁃HRMS.

关 键 词：猕猴桃根 结肠癌 化学成分 液质联用 

分 类 号：R917[医药卫生—药物分析学]