川芎嗪通过miR-34a对氧化型低密度脂蛋白诱导的冠状动脉内皮细胞损伤的保护作用  

Protective effect and mechanism of ligustrazine on coronary artery endothelial cell injury induced by oxidized low-density lipoprotein through miR-34a

作　　者：顾民华 顾勇 GU Minhua;GU Yong(Department of Cardiology,Hainan Provincial Hospital of Traditional Chinese Medicine,Haikou 570203,China)

机构地区：[1]海南省中医院心血管科,海南海口570203

出　　处：《陕西中医》2023年第9期1169-1172,共4页Shaanxi Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(82160899);海南省自然科学基金资助项目(821RC1128)。

摘　　要：目的:探讨川芎嗪是否通过miR-34a影响氧化型低密度脂蛋白(ox-LDL)诱导的人冠状动脉内皮细胞(HCAECs)损伤。方法:正常培养人HCAECs,作为Con组;用50 mg/L的ox-LDL干预HCAECs,建立细胞损伤模型,作为ox-LDL组。并设立1、10、100 mmol/L组(HCAECs用1、10、100 mmol/L川芎嗪处理+ox-LDL干预),ox-LDL+anti-miR-NC组、ox-LDL+anti-miR-34a组、ox-LDL+miR-NC组、ox-LDL+miR-34a组、ox-LDL+miR-NC+100 mmol/L组、ox-LDL+miR-34a+100 mmol/L组。分别采用试剂盒、酶联免疫吸附法(ELISA)和实时荧光定量PCR(RT-qPCR)检测HCAECs中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、肿瘤坏死因子-α(TNF-α)、白细胞介素1β(IL-1β)水平和微小核糖核酸34a(miR-34a)表达。结果:ox-LDL组HCAECs的MDA含量、TNF-α、IL-1β水平、miR-34a表达水平比Con组显著升高,SOD活力比Con组显著降低,差异有统计学意义(P<0.05);与ox-LDL组比较,川芎嗪显著降低细胞MDA含量、TNF-α、IL-1β水平以及miR-34a表达水平,显著升高SOD活力,差异有统计学意义(P<0.05)。与ox-LDL+anti-miR-NC组比较,ox-LDL+anti-miR-34a组细胞MDA含量以及TNF-α、IL-1β水平显著降低,SOD活力显著升高,差异有统计学意义(P<0.05)。ox-LDL+miR-34a+100 mmol/L组HCAECs的MDA含量以及TNF-α、IL-1β水平显著高于ox-LDL+miR-NC+100 mmol/L组,SOD活力显著低于ox-LDL+miR-NC+100 mmol/L组,差异有统计学意义(P<0.05)。结论:川芎嗪通过抑制miR-34a降低ox-LDL诱导的人HCAECs炎症反应和氧化应激。Objective:To investigate whether ligustrazine affects oxidized low-density lipoprotein(ox-LDL)-induced injury of human coronary artery endothelial cells(HCAECs)via miR-34a.Methods:Normally culture human HCAECs as the Con group.Intervene HCAECs with 50 mg/L ox-LDL to establish a cell injury model as the ox-LDL group.And set up 1,10,100 mmol/L groups(HCAECs were treated with 1,10,100 mmol/L ligustrazine+ox-LDL intervention),ox-LDL+anti-miR-NC group,ox-LDL+anti-miR-34a group,ox-LDL+miR-NC group,ox-LDL+miR-34a group,ox-LDL+miR-NC+100 mmol/L group,ox-LDL+miR-34a+100 mmol/L group.The Malondialdehyde(MDA)conten,Superoxide dismutase(SOD)activity,Tumor necrosis factor(TNF-α),Interleukin-1β(IL-1β)levels and miR-34a expression in HCAECs were detected by kits,ELISA and real-time quantitative PCR(RT-qPCR),respectively.Results:The MDA content,TNF-α,IL-1β,levels and miR-34a expression levels of HCAECs in the ox-LDL group were significantly higher than those in the Con group,and the SOD activity was significantly lower than those in the Con group(P<0.05).Compared with the ox-LDL group,ligustrazine significantly reduced the cell MDA,the content and the levels of TNF-αand IL-1β,significantly increased the activity of SOD(P<0.05).Compared with the ox-LDL+anti-miR-NC group,the cell MDA content,TNF-αand IL-1βlevels in the ox-LDL+anti-miR-34a group were significantly reduced,and the SOD activity was significantly increased(P<0.05).The MDA content,TNF-αand IL-1βlevels of HCAECs in the ox-LDL+miR-34a+100 mmol/L group were significantly higher than those in the ox-LDL+miR-NC+100 mmol/L group,and the SOD activity was significantly lower than that in the ox-LDL+miR-NC+100 mmol/L group(P<0.05).Conclusion:Ligustrazine can reduce ox-LDL-induced inflammatory response and oxidative stress in human HCAECs by inhibiting miR-34a.

关 键 词：川芎嗪 MIR-34A 人冠状动脉内皮细胞 炎症反应 氧化应激 氧化型低密度脂蛋白 

分 类 号：R541.4[医药卫生—心血管疾病]