扶正剔邪搜络方对肺纤维化大鼠血氧分压的影响  

Effect of Fuzheng Tixie Souluo Formula on Partial Pressure of Blood Oxygen in Pulmonary Fibrosis Rats

作　　者：练毅刚[1] 郑丰杰[2] 张宁[2] 温笑薇 张山 闫玉琴 崔兰凤 苏惠萍[1] LIAN Yigang;ZHENG Fengjie;ZHANG Ning;WEN Xiaowei;ZHANG Shan;YAN Yuqin;CUI Lanfeng;SU Huiping(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing China 100700;Beijing University of Chinese Medicine,Beijing China 100029;Beijing Longfu Hospital,Beijing China 100700)

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京中医药大学,北京100029 [3]北京市隆福医院,北京100700

出　　处：《中医学报》2023年第9期1961-1965,共5页Acta Chinese Medicine

基　　金：国家自然科学基金面上项目(81473657)。

摘　　要：目的:研究扶正剔邪搜络方对肺纤维化大鼠不同时相血氧分压的影响。方法:将健康清洁级雄性SD大鼠160只随机分为假手术组、模型组、扶正剔邪搜络方组、氧疗组、扶正剔邪搜络方+氧疗组,每组32只。除假手术组外,其余组大鼠气管内推注博莱霉素建立肺纤维化模型。造模24 h后,扶正剔邪搜络方组及扶正剔邪搜络方+氧疗组大鼠灌胃给予0.67 g·kg-1扶正剔邪搜络方颗粒剂溶液;吸氧组、假手术组和模型组灌胃给予相同体积的生理盐水,每天1次,连续28天。吸氧组和扶正剔邪搜络方+氧疗组大鼠每天吸氧2 h。给药第7、14、21、28天时,观察各组大鼠血氧分压变化;电镜下观察模型大鼠肺组织病理变化。结果:模型组肺小动脉内皮细胞肿胀,树状突起,凸向管腔,膜平滑肌增厚,内弹力板粗细不均匀,随着时间延长,大鼠肺泡纤维化越严重。与假手术组比较,第7、14、21、28天时,模型组血氧分压均明显降低(P<0.01);与模型组比较,第7、14、21、28天时,扶正剔邪搜络方组血氧分压均明显改善(P<0.01);第14天时,氧疗组血氧分压明显改善(P<0.01);第7、14、21天时,扶正剔邪搜络方+氧疗组血氧分压明显改善(P<0.05)。结论:扶正剔邪搜络方对肺纤维化大鼠血氧分压有明显的改善作用。Objective:To study the effect of Fuzheng Tixie Souluo Formula on partial pressure of blood oxygen in pulmonary fibrosis rats at different times.Methods:A total of 160 healthy and clean male SD rats were randomly divided into sham surgery group,model group,Fuzheng Tixie Souluo Formula group,oxygen therapy group,Fuzheng Tixie Souluo Formula+oxygen therapy group,with 32 rats in each group.Except for the sham surgery group,the rats in the remaining groups were injected with intratracheal bolus bleomycin to establish a pulmonary fibrosis model.After 24 h of molding,0.67 g·kg-1 of Fuzheng Tixie Souluo Formula Granule Solution was given to rats in the Fuzheng Elimination Evil Search Party group and Fuzheng+oxygen therapy group.The oxygen,sham and model groups were given the same volume of normal saline once a day for 28 days.Rats in the oxygen group and Fuzheng Tixie Souluo Formula+oxygen therapy group received oxygen for 2 h per day.On the 7th,14th,21st and 28th days of administration,the changes of partial pressure of oxygen in the rats were observed.Observation of lung histopathological changes in model rats under electron microscopy.Results:In the model group,the endothelial cells of the pulmonary arterioles were swollen,dendritic protrusions,convex to the lumen,the membrane smooth muscle thickened,the thickness of the internal elastic plate was uneven,and the more severe the alveolar fibrosis of rats became with prolonged time.Compared with the sham surgery group,the partial pressure of blood oxygen in the model group decreased significantly on days 7,14,21 and 28(P<0.01),compared with the model group,on the 7th,14th,21st and 28th days,the partial pressure of blood oxygen in the Fuzheng Tixie Souluo Formula Group was significantly improved(P<0.01),on the 14th day,the partial pressure of blood oxygen in the oxygen therapy group was significantly improved(P<0.01),and on the 7th,14th and 21st days,the partial pressure of blood oxygen in the Fuzheng Tixie Souluo Formula+Oxygen Therapy Group was significantly improved(

关 键 词：扶正剔邪搜络方 肺纤维化 血氧分压 大鼠 

分 类 号：R285.5[医药卫生—中药学]