启膈散增加食管癌对顺铂的敏感性与LncRNA MEG3的关系研究  

Relationship Between Increasing Sensitivity of Esophageal Cancer to Cisplatin with Qige Powder and LncRNAMEG3

作　　者：王静[1] 王美乐 尹素改[1] 冯书营 陈玉龙[1] 江华[1] 李亚兰[1] 李姝璇 WANG Jing;WANG Meile;YIN Sugai;FENG Shuying;CHEN Yulong;JIANG Hua;LI Yalan;LI Shuxuan(Henan University of Chinese Medicine,Zhengzhou Henan China 450046)

机构地区：[1]河南中医药大学,河南郑州450046

出　　处：《中医学报》2023年第9期1966-1974,共9页Acta Chinese Medicine

基　　金：河南省科技攻关项目(202102310504)。

摘　　要：目的:探讨启膈散增加食管癌对化疗药物顺铂敏感性的机制及与LncRNA MEG3的关系。方法:选用人食管癌细胞株EC9706,培养后加入不同剂量的启膈散及相应浓度的启膈散联合2 mg·L^(-1)顺铂,MTT法筛选启膈散的用药剂量并考察启膈散对EC9706细胞增殖的影响;流式细胞术检测细胞凋亡;Western Blot检测人第10号染色体缺失的磷酸酶及张力蛋白同源的基因(phosphates and tensin homologue deleted on chromosome ten gene,PTEN)蛋白表达水平;RT-PCR检测MEG3 mRNA、PTEN mRNA表达水平;细胞划痕实验和Transwell实验检测细胞迁移。结果:与对照组比较,启膈散可剂量依赖性抑制EC9706细胞的增殖(P<0.05),选取50 mg·L^(-1)启膈散及50 mg·L^(-1)启膈散与2 mg·L^(-1)顺铂联用进行后续研究。与对照组及顺铂组比较,启膈散组、启膈散联合顺铂组细胞凋亡率显著升高(P<0.01),PTEN蛋白表达量均显著升高(P<0.05),MEG3 mRNA表达量显著升高(P<0.01)。与对照组比较,启膈散组、顺铂组及启膈散联合顺铂组PTEN mRNA表达量显著升高(P<0.01),细胞迁移率显著降低(P<0.01)。与顺铂组比较,启膈散联合顺铂组PTEN mRNA表达量显著升高(P<0.05),细胞迁移率显著降低(P<0.01)。结论:启膈散干预EC9706细胞的增殖和迁移,促进凋亡,增加食管癌细胞对顺铂的敏感性,其机制可能与其上调MEG3进而影响PTEN信号通路有关。Objective:To explore the mechanism of Qige Powder increasing the sensitivity of esophageal cancer to chemotherapy drug cisplatin and its relationship with LncRNAMEG3.Method:The human esophageal cancer cell line EC9706 was selected for the experiment.After cultivation,different doses of Qige Powder and corresponding concentrations of Qige Powder combined with 2 mg·L^(-1)cisplatin were added.The drug dosage of Qige Powder was screened by MTT method and the effect of Qige Powder on EC9706 cell proliferation was investigated;Flow cytometry was used to detect cell apoptosis;Western blot was used to detect the protein expression level of phosphatase and tensin homologue deleted from chromosome Chromosome 10;RT-PCR was used to detect the expression levels of MEG3 mRNA and PTEN mRNA;Cell scratch test and Transwell test were used to detect Cell migration.Results:Compared with the control group,Qige San could inhibit the proliferation of EC9706 cells in a dose-dependent manner(P<0.05).50 mg·L^(-1)Qige San and 50 mg·L^(-1)Qige San combined with 2 mg·L^(-1)cisplatin were selected for follow-up study.Compared with the control group and cisplatin group,the apoptosis rate of cells in the Qige San group and the Qige Powder combined with cisplatin group were significantly increased(P<0.01),the expression of PTEN protein was significantly increased(P<0.05),and the expression of MEG3 mRNA was significantly increased(P<0.01).Compared with the control group,the expression of PTEN mRNA in Qige Powder group,cisplatin group and Qige Powder plus cisplatin group increased significantly(P<0.01),and the Cell migration rate decreased significantly(P<0.01).Compared with cisplatin group,the expression of PTEN mRNA in Qige Powder combined with cisplatin group was significantly higher(P<0.05),and the Cell migration rate was significantly lower(P<0.01).Conclusion:Qige Powder interferes with the proliferation and migration of EC9706 cells,promotes apoptosis,and increases the sensitivity of esophageal cancer cells to cisplatin.Its mechanism may

关 键 词：食管癌 启膈散 顺铂 LncRNA MEG3 PTEN信号通路 

分 类 号：R285.5[医药卫生—中药学]