积雪草酸对前列腺癌细胞DU145的作用研究  

Study on the effect of Asiatic acid on prostate cancer cells DU145

作　　者：刘嘉 严宝飞 田朝晖 曾庆琪[2] LIU Jia;YAN Baofei;TIAN Zhaohui;ZENG Qingqi(College of Pharmacy,Jiangsu Health Vocational College,211800 Nanjing,China;The First Clinical Medical College,Nanjing University of Chinese Medicine,210046 Nanjing,China)

机构地区：[1]江苏卫生健康职业学院药学院,江苏南京211800 [2]南京中医药大学第一临床医学院,江苏南京210046

出　　处：《中国肿瘤外科杂志》2023年第4期391-395,共5页Chinese Journal of Surgical Oncology

基　　金：江苏省卫生健康委医学科研项目(ZDB2020020);江苏省高校“青蓝工程”优秀教学团队培养对象(苏教师[2020]42号)。

摘　　要：目的研究积雪草酸(AA)对前列腺癌DU145细胞增殖和凋亡的影响。方法以不同浓度AA进行药物干预DU145细胞,MTT法检测细胞活力变化;显微镜观察细胞形态改变;Hoechst染色及Annexin V/PI双染法检测细胞凋亡;Western Blot法检测细胞线粒体凋亡相关蛋白表达变化;试剂盒检测细胞Caspase 3活性变化。结果与空白组比较,AA能够有效抑制前列腺癌DU145细胞的增殖,显著诱导细胞凋亡(P<0.01),显著提升细胞Bax、Cleaved Caspase 3、Cyt-C蛋白表达和Bax/Bcl-2比值(P<0.05,P<0.01),显著降低Bcl-2蛋白表达(P<0.05,P<0.01),显著促进细胞Caspase 3活化(P<0.05,P<0.01)。与AA组比较,Caspase 3抑制剂Z-VAD-FMK能够显著抑制AA诱导的细胞Caspase 3活化(P<0.05),显著逆转AA诱导的细胞增殖抑制(P<0.05,P<0.01)。结论AA能够通过抑制DU145细胞增殖及诱导线粒体途径凋亡发挥抗前列腺癌作用。Objective To investigate the effect of Asiatic acid(AA)on prostate cancer DU145 cell growth and apoptosis.Methods DU145 cells were intervened with different concentrations of AA,and the changes of cell viability were detected by MTT;The changes of cell morphology were observed by microscopy;Apoptosis was detected by Hoechst staining and Annexin V/PI double-staining;The changes of mitochondrial apoptosis-related protein expression were detected by Western Blot;The changes of cell Caspase 3 activity were detected by the kit.Results Compared with the control group,AA could effectively inhibit the proliferation of prostate cancer DU145 cells,significantly induce apoptosis(P<0.01),significantly increase cellular Bax,Cleaved Caspase 3,Cyt-C protein expression and Bax/Bcl-2 ratio(P<0.05,P<0.01),significantly decrease Bcl-2 protein expression(P<0.05,P<0.01),and significantly promote cellular Caspase 3 activation(P<0.05,P<0.01).Compared with the AA group,the Caspase 3 inhibitor Z-VAD-FMK significantly inhibited AA-induced cellular Caspase 3 activation(P<0.05)and significantly reversed AA-induced cell proliferation inhibition(P<0.05,P<0.01).Conclusions AA can exert anti-prostate cancer effects by inhibiting DU145 cell proliferation and inducing the mitochondrial pathway of apoptosis.

关 键 词：积雪草酸 前列腺癌 DU145细胞 细胞增殖 线粒体凋亡 

分 类 号：R285[医药卫生—中药学]