多重PCR技术在植物病原物检测中的应用  

Application of Multiplex PCR on Detection of Plant Pathogens

作　　者：曹子健 邱艳红 王爽 赵娟[1] 郑素月 乔广行[1] 秦文韬 CAO Zijian;QIU Yanhong;WANG Shuang;ZHAO Juan;ZHENG Suyue;QIAO Guanghang;QIN Wentao(Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;School of Landscape and Ecological Engineering,Hebei University of Engineering,Hebei Handan 056038,China;Institute of Vegetable Science,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Administrative Office of the Summer Palace,Beijing Municipal Administration Center of Parks,Beijing 100097,China)

机构地区：[1]北京市农林科学院植物保护研究所,北京100097 [2]河北工程大学园林与生态工程学院,河北邯郸056038 [3]北京市农林科学院蔬菜研究所,北京100097 [4]北京市颐和园管理处,北京100097

出　　处：《中国农业科技导报》2023年第8期216-224,共9页Journal of Agricultural Science and Technology

基　　金：北京市农林科学院创新能力建设专项(KJCX20220415);财政部与农业农村部国家桃产业技术体系建设项目(CARS-30);北京市乡村振兴项目(BJXCZX20221229)。

摘　　要：植物病原物种类繁多,且气候变化及全球化发展加剧了病原的扩散。快速可靠的植物病原诊断技术对病害的流行预警和防控管理具有重要作用。随着技术发展,多重PCR技术因具有精准、高效的特点而被广泛应用到生命科学的多个领域。对多重PCR技术的原理及其在植物病原真菌、细菌、病毒、线虫检测中的应用进行了系统综述,分析了该技术目前存在的问题及解决方式,并展望了该技术未来的发展前景。There are various plant pathogens,and climate change and globalization have intensified the spread of pathogens.Rapid and reliable diagnostic techniques for plant pathogens play important roles for the epidemic warning and scientific prevention of the disease.With the development of technology,multiplex PCR technology with the advantages of high accuracy and efficiency is widely used in many fields of the life sciences.The principle of this technical was briefly introduced,and the applications of multiplex PCR in the detection of plant pathogens including fungi,bacteria,viruses and nematodes were also reviewed.The existing problems and solutions of this technology were analyzed,and the development of this technology in the future was finally prospected.

关 键 词：多重PCR 植物病原物 检测 应用 

分 类 号：S432[农业科学—植物病理学]